Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Chicken Cell (retina)
Specification
retina
Permeabilization
Yes - triton X-100 in PBS
Fixative
Paraformaldehyde
Other product details
Concentration
1 µg/ml
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS + 0.02% triton X-100
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen Alexa Fluor goat anti-rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
left image: mouse;
middle image: guinea pig;
right image: chicken.
Abcam response
Thank you so much for testing this antibody in guinea pig and chicken.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in these species. The customer received a discount on a future antibody purchase for testing this product in guinea pig and chicken. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Chris Zelinka
Verified customer
提交于 Jun 04 2013