Anti-Glucagon Receptor抗体(ab75240)
Key features and details
- Rabbit polyclonal to Glucagon Receptor
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Glucagon Receptor抗体
参阅全部 Glucagon Receptor 一抗 -
描述
兔多克隆抗体to Glucagon Receptor -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthesized peptide derived from internal of human Glucagon Receptor
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阳性对照
- extracts from COLO205 cells; HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75240于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 54 kDa.
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ICC/IF |
1/500 - 1/1000.
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IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/500 - 1/1000. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2642 Human
- Entrez Gene: 14527 Mouse
- Entrez Gene: 24953 Rat
- Omim: 138033 Human
- SwissProt: P47871 Human
- SwissProt: Q61606 Mouse
- SwissProt: P30082 Rat
- Unigene: 208 Human
see all -
别名
- Gcgr antibody
- GGR antibody
- GL R antibody
see all
图片
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All lanes : Anti-Glucagon Receptor antibody (ab75240) at 1/500 dilution
Lane 1 : extracts from COLO205 cells
Lane 2 : extracts from COLO205 cells with immunizing peptide at 5 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75240. Right panel: same, after pre-incubation with the immunizing peptide.
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IHC image of ab75240 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75240, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab75240 被引用在 1 文献中.
- Quiñones M et al. Hypothalamic CaMKKß mediates glucagon anorectic effect and its diet-induced resistance. Mol Metab 4:961-70 (2015). WB . PubMed: 26909312