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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels Glycine Receptor

Anti-GLRA2抗体(ab97628)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (3)

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Western blot - Anti-GLRA2 antibody (ab97628)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLRA2 antibody (ab97628)

Key features and details

  • Rabbit polyclonal to GLRA2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-GLRA2抗体
  • 描述

    兔多克隆抗体to GLRA2
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant fragment corresponding to Human GLRA2 aa 1-186 (N terminal).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • Glycine Receptor

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97628于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P
1/100 - 1/500.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P
1/100 - 1/500.

靶标

  • 功能

    The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
  • 序列相似性

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA2 sub-subfamily.
  • 细胞定位

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Target information above from: UniProt accession P23416 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2742 Human
    • Entrez Gene: 237213 Mouse
    • Entrez Gene: 24397 Rat
    • Omim: 305990 Human
    • SwissProt: P23416 Human
    • SwissProt: Q7TNC8 Mouse
    • SwissProt: P22771 Rat
    • Unigene: 2700 Human
    • Unigene: 113877 Mouse
    • Unigene: 10379 Rat
    see all
  • 别名

    • GLR antibody
    • Glra2 antibody
    • GLRA2_HUMAN antibody
    • Glycine receptor alpha 2 polypeptide antibody
    • Glycine receptor alpha 2 subunit antibody
    • Glycine receptor subunit alpha 2 antibody
    • Glycine receptor subunit alpha-2 antibody
    • Gycine receptor alpha 2 antibody
    see all

图片

  • Western blot - Anti-GLRA2 antibody (ab97628)
    Western blot - Anti-GLRA2 antibody (ab97628)
    Anti-GLRA2 antibody (ab97628) at 1/5000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    10 % SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLRA2 antibody (ab97628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLRA2 antibody (ab97628)

    ab97628, at a 1/500 dilution, staining GLRA2 in paraffin embedded MDAMB468 xenograft by Immunohistochemistry.

实验方案

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (3)

发表研究结果有使用 ab97628?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab97628 被引用在 3 文献中.

  • Qi M  et al. Transient Receptor Potential Vanilloid 4 Activation-Induced Increase in Glycine-Activated Current in Mouse Hippocampal Pyramidal Neurons. Cell Physiol Biochem 45:1084-1096 (2018). PubMed: 29439248
  • Förstera B  et al. Presence of ethanol-sensitive glycine receptors in medium spiny neurons in the mouse nucleus accumbens. J Physiol 595:5285-5300 (2017). PubMed: 28524260
  • Ren M  et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep 6:24953 (2016). ICC/IF ; Rat . PubMed: 27112667

客户评价及客户问答

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Question

I'm seeing multiple bands in WB using this antibody and mouse skin tissue.  I'm seeing bands around 40 & 60 kDa, but nothing around 50 kDa. I would also like to test ab97628 in mouse, an untested species.

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Abcam community

Verified customer

Asked on Nov 18 2011

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab80972. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. For testing ab97628, please see the information below.   I am very pleased to hear you would like to accept our offer and test ab97628 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.    

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Abcam Scientific Support

回复于 Nov 18 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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