Anti-GLO1抗体(ab96032)
Key features and details
- Rabbit polyclonal to GLO1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GLO1抗体
参阅全部 GLO1 一抗 -
描述
兔多克隆抗体to GLO1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow
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免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 and 171 of GLO1 (NP_006699).
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阳性对照
- WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT and Raji cell lysates IHC-P: ovarian carcinoma tissue ICC/IF: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
|
|
| IHC-P | (2) |
1/100 - 1/250.
|
| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
|
IHC-P
1/100 - 1/250. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. -
通路
Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2. -
序列相似性
Belongs to the glyoxalase I family. - Information by UniProt
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数据库链接
- Entrez Gene: 2739 Human
- Entrez Gene: 109801 Mouse
- Omim: 138750 Human
- SwissProt: Q04760 Human
- SwissProt: Q9CPU0 Mouse
- Unigene: 268849 Human
- Unigene: 261984 Mouse
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别名
- Aldoketomutase antibody
- glo1 antibody
- GLOD1 antibody
see all
图片
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Western blot - Anti-GLO1 antibody (ab96032)All lanes : Anti-GLO1 antibody (ab96032) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO1 antibody (ab96032)Immunohistochemical analysis of GLO1 in paraffin embedded human ovarian carcinoma tissue, using ab96032 at a 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-GLO1 antibody (ab96032)Immunofluorescence analysis of GLO1 in paraformaldehyde fixed HeLa, using ab96032 at a 1/200 dilution. Lower image: merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab96032 被引用在 15 文献中.
- Jandova J & Wondrak GT Genomic GLO1 deletion modulates TXNIP expression, glucose metabolism, and redox homeostasis while accelerating human A375 malignant melanoma tumor growth. Redox Biol 39:101838 (2021). PubMed: 33360689
- Rounds L et al. Glyoxalase 1 Expression as a Novel Diagnostic Marker of High-Grade Prostatic Intraepithelial Neoplasia in Prostate Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34298821
- Monteiro-Alfredo T et al. Hypoglycaemic and Antioxidant Properties of Acrocomia aculeata (Jacq.) Lodd Ex Mart. Extract Are Associated with Better Vascular Function of Type 2 Diabetic Rats. Nutrients 13:N/A (2021). PubMed: 34445015
- Rodrigues T et al. GLP-1 improves adipose tissue glyoxalase activity and capillarization improving insulin sensitivity in type 2 diabetes. Pharmacol Res 161:105198 (2020). PubMed: 32942016
- Jandova J et al. Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo. Cancers (Basel) 12:N/A (2020). PubMed: 32466621