Anti-GLO1抗体[6F10] (ab81461)
Key features and details
- Rat monoclonal [6F10] to GLO1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG2b
概述
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产品名称
Anti-GLO1抗体[6F10]
参阅全部 GLO1 一抗 -
描述
大鼠单克隆抗体[6F10] to GLO1 -
宿主
Rat -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human, African green monkey -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: COS-1, L929 and HeLa whole cell extracts. ICC/IF: Hela cells. HeLa cells : Flow Cyt (Intra).
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常规说明
ab81461 also reacts with Simian.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯化说明
The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. It is filter sterilized. -
Primary antibody说明
ab81461 also reacts with Simian. -
克隆
单克隆 -
克隆编号
6F10 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81461于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 21 kDa.
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说明 |
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Flow Cyt (Intra)
Use 1µg for 106 cells. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 21 kDa. |
靶标
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功能
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. -
通路
Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2. -
序列相似性
Belongs to the glyoxalase I family. - Information by UniProt
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数据库链接
- Entrez Gene: 2739 Human
- Entrez Gene: 109801 Mouse
- Omim: 138750 Human
- SwissProt: Q04760 Human
- SwissProt: Q9CPU0 Mouse
- Unigene: 268849 Human
- Unigene: 261984 Mouse
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别名
- Aldoketomutase antibody
- glo1 antibody
- GLOD1 antibody
see all
图片
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All lanes : Anti-GLO1 antibody [6F10] (ab81461) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GLO1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDaLanes 1 - 4: Merged signal (red and green). Green - ab81461 observed at 21 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab81461 was shown to specifically react with in wild-type HAP1 cells as signal was lost in GLO1 knockout cells. Wild-type and GLO1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab81461 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-GLO1 antibody [6F10] (ab81461)
Lane 1 : COS-1 (simian) whole cell extract
Lane 2 : L929 (mouse) whole cell extract
Lane 3 : HeLa (human) whole cell extract
Predicted band size: 21 kDa
Observed band size: 27-29 kDa why is the actual band size different from the predicted?
Mouse GLO1 shows a single band of 27 kDa while human and simian ones show 29 kDa. -
Immunofluorescent staining of HeLa cells with ab81461.
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Overlay histogram showing HeLa cells stained with ab81461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81461, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab81461 被引用在 11 文献中.
- Lodd E et al. The combination of loss of glyoxalase1 and obesity results in hyperglycemia. JCI Insight 4:N/A (2019). PubMed: 31217350
- Schumacher D et al. Compensatory mechanisms for methylglyoxal detoxification in experimental & clinical diabetes. Mol Metab 18:143-152 (2018). PubMed: 30287091
- Morgenstern J et al. Loss of Glyoxalase 1 Induces Compensatory Mechanism to Achieve Dicarbonyl Detoxification in Mammalian Schwann Cells. J Biol Chem 292:3224-3238 (2017). PubMed: 27956549
- Salvi A et al. Protective Effect of Tempol on Buthionine Sulfoximine-Induced Mitochondrial Impairment in Hippocampal Derived HT22 Cells. Oxid Med Cell Longev 2016:5059043 (2016). WB . PubMed: 27069531
- Patki G et al. Tempol treatment reduces anxiety-like behaviors induced by multiple anxiogenic drugs in rats. PLoS One 10:e0117498 (2015). WB ; Rat . PubMed: 25793256