Key features and details
- Goat polyclonal to GIPC1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
经测试应用适用于: IHC-P, WBmore details
预测可用于: Mouse, Rat
常规说明GenBank Accession Number – NP_005707
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab5951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
1 hour primary incubation is recommended for this product.
相关性GIPC1 is a hydrophilic protein that contains 333 amino acids, including multiple phosphorylation sites and an 80 to 100 amino acid PDZ domain. PDZ domain-containing proteins typically recognize C terminal amino acids and are involved in protein network signaling. GIPC1 may be involved in G protein-linked signaling.
细胞定位Cell Membrane, peripheral membrane protein and Cytoplasmic.
- C19ORF3 antibody
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ab5951 at 1
µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL).
Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size: 38 kDa
Observed band size: 24,38 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.
ab5951 被引用在 4 文献中.
- Henkel B et al. NHERF1 in Microvilli of Vomeronasal Sensory Neurons. Chem Senses 42:25-35 (2017). PubMed: 27655939
- Wu D et al. GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. J Biol Chem 285:28643-50 (2010). ICC/IF ; Human . PubMed: 20634288
- Valdembri D et al. Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol 7:e25 (2009). WB ; Human . PubMed: 19175293
- Zerbinatti CV et al. Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein. Mol Neurodegener 3:5 (2008). WB ; Human . PubMed: 18348724