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Signal Transduction Signaling Pathway G Protein Signaling GPCR

Anti-GIPC1抗体(ab5951)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (4)

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Western blot - Anti-GIPC1 antibody (ab5951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
  • Western blot - Anti-GIPC1 antibody (ab5951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)

Key features and details

  • Goat polyclonal to GIPC1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-GIPC1抗体
  • 描述

    山羊多克隆抗体to GIPC1
  • 宿主

    Goat
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human GIPC1 aa 300 to the C-terminus (C terminal). (NP_974197.1; NP_974199.1; NP_974196.1; NP_974198.1; NP_974223.1)
    Database link: O14908

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human GIPC1 protein (ab96769)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5951于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
Use at an assay dependent concentration.
WB (2)
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).

1 hour primary incubation is recommended for this product.

说明
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    May be involved in G protein-linked signaling.
  • 组织特异性

    Widely expressed.
  • 序列相似性

    Belongs to the GIPC family.
    Contains 1 PDZ (DHR) domain.
  • 细胞定位

    Cytoplasm. Membrane.
  • Target information above from: UniProt accession O14908 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10755 Human
    • Entrez Gene: 67903 Mouse
    • Entrez Gene: 83823 Rat
    • Omim: 605072 Human
    • SwissProt: O14908 Human
    • SwissProt: Q9Z0G0 Mouse
    • SwissProt: Q9Z254 Rat
    • Unigene: 655012 Human
    • Unigene: 741240 Human
    • Unigene: 20945 Mouse
    • Unigene: 30044 Rat
    see all
  • 别名

    • C19ORF3 antibody
    • Chromosome 19 open reading frame 3 antibody
    • GAIP C-terminus-interacting protein antibody
    • GIPC antibody
    • GIPC PDZ domain containing family member 1 antibody
    • GIPC1 antibody
    • GIPC1_HUMAN antibody
    • GLUT1 C terminal binding protein antibody
    • GLUT1CBP antibody
    • Hs.6454 antibody
    • IGF 1 receptor interacting protein 1 antibody
    • IIP 1 antibody
    • IIP1 antibody
    • NIP antibody
    • PDZ domain-containing protein GIPC1 antibody
    • Regulator of G protein signalling 19 interacting protein 1 antibody
    • RGS-GAIP-interacting protein antibody
    • RGS19 interacting protein 1 antibody
    • RGS19-interacting protein 1 antibody
    • RGS19IP1 antibody
    • SEMCAP antibody
    • SYNECTIIN antibody
    • Synectin antibody
    • Tax interaction protein 2 antibody
    • TIP 2 antibody
    • TIP-2 antibody
    see all

图片

  • Western blot - Anti-GIPC1 antibody (ab5951)
    Western blot - Anti-GIPC1 antibody (ab5951)
    ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
    Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.

    Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • Western blot - Anti-GIPC1 antibody (ab5951)
    Western blot - Anti-GIPC1 antibody (ab5951)
    Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size: 38 kDa
    Observed band size: 24,38 kDa why is the actual band size different from the predicted?



    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)This image is courtesy of an anonymous Abreview
    ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.

    See Abreview

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (4)

发表研究结果有使用 ab5951?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5951 被引用在 4 文献中.

  • Henkel B  et al. NHERF1 in Microvilli of Vomeronasal Sensory Neurons. Chem Senses 42:25-35 (2017). PubMed: 27655939
  • Wu D  et al. GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. J Biol Chem 285:28643-50 (2010). ICC/IF ; Human . PubMed: 20634288
  • Valdembri D  et al. Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol 7:e25 (2009). WB ; Human . PubMed: 19175293
  • Zerbinatti CV  et al. Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein. Mol Neurodegener 3:5 (2008). WB ; Human . PubMed: 18348724

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-GIPC1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (ventral striatum)
Gel Running Conditions
Non-reduced Denaturing (10% TGX)
Loading amount
30 µg
Specification
ventral striatum
Blocking step
licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Jul 11 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GIPC1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (prostate tissue section)
Specification
prostate tissue section
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Read More

Abcam user community

Verified customer

提交于 Oct 15 2009

Western blot abreview for Anti-GIPC1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Arterial endothelial cells)
Loading amount
30 µg
Specification
Arterial endothelial cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Read More

Mrs. Elena Astanina

Verified customer

提交于 Apr 09 2008

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