Anti-GIPC1抗体(ab5951)
Key features and details
- Goat polyclonal to GIPC1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GIPC1抗体 -
描述
山羊多克隆抗体to GIPC1 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide: GAIGDAKVGRY, corresponding to C terminal amino acids 323-333 of Human GIPC1.
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常规说明
GenBank Accession Number – NP_005707
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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相关性
GIPC1 is a hydrophilic protein that contains 333 amino acids, including multiple phosphorylation sites and an 80 to 100 amino acid PDZ domain. PDZ domain-containing proteins typically recognize C terminal amino acids and are involved in protein network signaling. GIPC1 may be involved in G protein-linked signaling. -
细胞定位
Cell Membrane, peripheral membrane protein and Cytoplasmic. -
数据库链接
- Entrez Gene: 10755 Human
- Entrez Gene: 67903 Mouse
- Entrez Gene: 83823 Rat
- Omim: 605072 Human
- SwissProt: O14908 Human
- SwissProt: Q9Z0G0 Mouse
- SwissProt: Q9Z254 Rat
- Unigene: 655012 Human
see all -
别名
- C19ORF3 antibody
- Chromosome 19 open reading frame 3 antibody
- GAIP C-terminus-interacting protein antibody
see all
图片
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Western blot - Anti-GIPC1 antibody (ab5951)ab5951 at 1µ g/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Western blot - Anti-GIPC1 antibody (ab5951)Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size: 38 kDa
Observed band size: 24,38 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)This image is courtesy of an anonymous Abreviewab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.
实验方案
文献 (4)
ab5951 被引用在 4 文献中.
- Henkel B et al. NHERF1 in Microvilli of Vomeronasal Sensory Neurons. Chem Senses 42:25-35 (2017). PubMed: 27655939
- Wu D et al. GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. J Biol Chem 285:28643-50 (2010). ICC/IF ; Human . PubMed: 20634288
- Valdembri D et al. Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol 7:e25 (2009). WB ; Human . PubMed: 19175293
- Zerbinatti CV et al. Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein. Mol Neurodegener 3:5 (2008). WB ; Human . PubMed: 18348724
