Anti-GIPC1抗体(ab5951)
Key features and details
- Goat polyclonal to GIPC1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GIPC1抗体 -
描述
山羊多克隆抗体to GIPC1 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide: GAIGDAKVGRY, corresponding to C terminal amino acids 323-333 of Human GIPC1.
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常规说明
GenBank Accession Number – NP_005707Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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相关性
GIPC1 is a hydrophilic protein that contains 333 amino acids, including multiple phosphorylation sites and an 80 to 100 amino acid PDZ domain. PDZ domain-containing proteins typically recognize C terminal amino acids and are involved in protein network signaling. GIPC1 may be involved in G protein-linked signaling. -
细胞定位
Cell Membrane, peripheral membrane protein and Cytoplasmic. -
数据库链接
- Entrez Gene: 10755 Human
- Entrez Gene: 67903 Mouse
- Entrez Gene: 83823 Rat
- Omim: 605072 Human
- SwissProt: O14908 Human
- SwissProt: Q9Z0G0 Mouse
- SwissProt: Q9Z254 Rat
- Unigene: 655012 Human
see all -
别名
- C19ORF3 antibody
- Chromosome 19 open reading frame 3 antibody
- GAIP C-terminus-interacting protein antibody
see all
图片
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Western blot - Anti-GIPC1 antibody (ab5951)ab5951 at 1
µ g/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Western blot - Anti-GIPC1 antibody (ab5951)Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size: 38 kDa
Observed band size: 24,38 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)This image is courtesy of an anonymous Abreviewab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.
实验方案
文献 (4)
ab5951 被引用在 4 文献中.
- Henkel B et al. NHERF1 in Microvilli of Vomeronasal Sensory Neurons. Chem Senses 42:25-35 (2017). PubMed: 27655939
- Wu D et al. GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. J Biol Chem 285:28643-50 (2010). ICC/IF ; Human . PubMed: 20634288
- Valdembri D et al. Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol 7:e25 (2009). WB ; Human . PubMed: 19175293
- Zerbinatti CV et al. Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein. Mol Neurodegener 3:5 (2008). WB ; Human . PubMed: 18348724
