概述

  • 产品名称

    GFP ELISA试剂盒
  • 检测方法

    Colorimetric
  • 精确度

    批次内
    样品 n Mean SD CV%
    Cell Lysate 9 3.1%
    批次间
    样品 n Mean SD CV%
    Cell Lysate 3 8.2%
  • 样品类型

    Cell Lysate, Cell culture media, Tissue Lysate
  • 检测类型

    Sandwich (quantitative)
  • 灵敏度

    1.8 pg/ml
  • 范围

    2.7 pg/ml - 2000 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Cell culture media 102 101% - 103%
    Fetal Bovine Serum 97 96% - 98%

  • 检测时间

    1h 30m
  • 实验步骤

    One step assay
  • 产品概述

    GFP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in cell and tissue lysates. Quantitate human GFP with 1.8 pg/ml sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.


    This kit will detect both the Enhanced and Superfold variants of GFP.

  • 说明

    Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

  • 经测试应用

    适用于: Sandwich ELISAmore details
  • 平台

    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab171581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • This kit recognizes both GFP and enhanced GFP (eGFP) in cell and tissue extracts. No reactivity with mCherry, RFP or YFP was observed.

  • Background subtracted data from triplicate measurements are plotted.

实验方案

文献

This product has been referenced in:

  • Lin CJ  et al. Microbubble-facilitated ultrasound pulsation promotes direct a-synuclein gene delivery. Biochem Biophys Res Commun 517:77-83 (2019). Read more (PubMed: 31327496) »
  • Hori A  et al. Unexpected role of the IMD pathway in Drosophila gut defense against Staphylococcus aureus. Biochem Biophys Res Commun 495:395-400 (2018). Read more (PubMed: 29108998) »
See all 5 Publications for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Question
Answer

We have not tested ab171581 with a GFP fusion protein (purified or in cell extract) before. The epitopes for the capture and detector antibodies are also not known because both are made using full length immunogens. However, both antibodies alone have detected GFP fusion proteins in other applications (WB, IP), so it is encouraging that at least the individual antibodies alone are capable of detecting GFP fusion proteins.

Read More

Test for superfold GFP

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The kit works with superfold GFP. I have tested again our purified superfold GFP protein and also some cell lysate from cells expressing superfold GFP. Both worked.

When compared with the GFP stock provided by the kit, the sensitivity against superfold GFP is lower than GFP. This could be due to our protein stock is stored at -80C for sometime.

Ling Chun

Verified customer

提交于 Oct 27 2014

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