Application
Western blot
Sample
Human Cell lysate - whole cell (hek293)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Treatment
transfected with GFP-cDNA
Specification
hek293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: fluorescent secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 790
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 790
Detection
Detection method
Fluorescense
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 70, 100, 120 kDa
Positive control
GFP-fused cDNAs at various sizes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 12 2018