Anti-GFP抗体(ab6556)

Regionally restricted CX3CL1 expression in the retina of adult CX3CL1 cherry: CX3CR1gfp mouse (A-E)

Fluorescent light microscopy analysis of a vertical retina cryosection (A) showing both CX3CL1/Cherry (detected in rhodamine channel, red) (B) and CX3CR1/GFP (detected in FITC channel, green) (C) reporters expression. CX3CR1/GFP signal is assigned to microglial cells with cell bodies and dendritic ramifications in three sublayers (outer plexiform layer, OPL, inner plexiform layer, IPL and ganglion cells layer, GCL). CX3CL1/Cherry fluorescent cells are found exclusively in ganglion cell and inner nuclear layer. No fluorescent cells were observed in outer nuclear layer (ONL). Enlargements show strong Cherry signal in a presumable amacrine cell body in inner nuclear (D, arrow points to dendritic base) and ganglion cell (E) layers, indicating high level of the CX3CL1 chemokine promotor activity in neurons. Cryosections are counterstained with DAPI nucleic acid stain (blue). Distribution of the Cherry reporter positive cells in the retina of CX3CL1cherry: CX3CR1gfp mice coincides mostly with results obtained in in situ hybridization studies. Of note, membrane/lipid inner and outer photoreceptor segment layer exhibit nonspecific fluorescence. Scale bars represent 50 µm (A, B and C), and 10 µm (D, E).

GFP was detected with ab6556 at 1/1000 dilution.

(From Figure 4 of Zieger et al)

The downstream Wnt non-canonical pathway components are required in the escort cells for cystoblast encapsulation

(A-D1) Germaria of c587-GAL4, RhoA, Rac1 and cdc42 depleted escort cells stained with Tj (red), GFP (green) and Vasa (blue) showing loss of encapsulation in RhoA, Rac1 and cdc42 depleted escort cells. Fax-GFP marks somatic cell membranes. GFP channel is shown in A1, B1, C1 and D1. Scale bar for all images is 20μm.

GFP is detected with ab6556.

(After Figure 2 of Upadhyay et al)

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ab6556 staining GFP in mouse tooth tissue section by Immunohistochemistry (Frozen sections) without fixation. Tissue samples were blocked with 1% BSA for 20 minutes at 20^oC. The sample was incubated with primary antibody (1/500) for 2 hours at 20^oC. An Alexa Fluor^®488-conjugated Chicken polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution. Immunofluorescent localization of CD31 and GFP in implanted non GFP cultured molars in GFP adult mice showed the origin of neo-formed blood vessels.
DP: Dental Pulp
EO: Enamel Organ

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This image shows a single primary hippocampal neuron from a primary culture overexpressing GFP stained with ab6556 at a dilution of 1/2000. This picture was kindly supplied as part of the review submitted by one of our customers.

This image shows IF using GFP-expressing glial cells (green) transplanted into lesioned rat spinal cord. This was detected using ab6556 anti-GFP antibody and a FITC conjugated secondary antibody. Axons are labelled red by an antibody to neurofilament-200 and a rhodamine secondary. ab6556 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious. The top picture shows an optical section from a confocal microscope scan showing how a GFP cell wraps around a branched axon travelling longitudinally. The bottom picture consists of an optical section from another confocal scan showing a GFP cell enveloping an axon in the transverse plane. Review by Andrew Toft submitted 19 May 2004.

ab6556 at 1/500 dilution staining GFP in mouse testis by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed prior to blocking in 100% serum for 1 hour at 37°C and then incubated with ab6556 for 1 hour at 37°C. A Texas Red conjugated chicken polyclonal to rabbit Ig, diluted 1/100, was used as the secondary antibody.

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Specific labeling of a Trk-GFP fusion protein being synthesized on ER in sympathetic neurons infected with an adenovirus carrying the construct. The gold is associated with the ER membranes. This was done using a 1/5000 dilution of affinity purified antibody (ab6556). The tissue section was fixed and embedded using durcupan resin.