Anti-GFP抗体(ab5449)

Reviews (1)Q&A (4)References (10)
Western blot - Anti-GFP antibody (ab5449)
  • Western blot - Anti-GFP antibody (ab5449)

Key features and details

  • Goat polyclonal to GFP
  • Suitable for: WB, IP
  • Reacts with: Species independent
  • Isotype: IgG

概述

  • 产品名称

    Anti-GFP抗体
    参阅全部 GFP 一抗

  • 描述

    山羊多克隆抗体to GFP

  • 宿主

    Goat

  • 特异性

    Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    This information is considered to be commercially sensitive.

  • 阳性对照

    • Pure GFP protein, or cells known to overexpress GFP.

  • 常规说明

    Protein A will not bind goat IgG, so use alternates (eg. protein G) in IP with this antibody. This antibody is available in an affinity purified form as ab5450.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    Preservative: 0.05% Sodium azide
  • Concentration information loading...

  • 纯度

    Whole antiserum

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5449于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/5000 - 1/20000.
IP
Use 0.5µl for 106 cells.
说明
WB
1/5000 - 1/20000.
IP
Use 0.5µl for 106 cells.

靶标

  • 相关性

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.

  • 别名

    • GFP antibody
    • Green fluorescent protein antibody

图片

  • Western blot - Anti-GFP antibody (ab5449)
    Western blot - Anti-GFP antibody (ab5449)

    Lane 1       :  parental YTS cells (negative control)
    Lanes 2-5 :  YTS cells transfected with KIR-EGFP (mw 88 kD)

    KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33: 1084).

     



    Lane 1 : parental YTS cells (negative control) Lanes 2-5 : YTS cells transfected with KIR-EGFP (mw 88 kD) KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33: 1084).
  • Western blot - Anti-GFP antibody (ab5449)
    Western blot - Anti-GFP antibody (ab5449)

    5 ng GFP on  PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of:
    Lane 1 : 1/2500
    Lane 2 : 1/5000
    Lane 3 :  1/10,000
    Lane 4 :  1/20,000



    5 ng GFP on PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of: Lane 1 : 1/2500 Lane 2 : 1/5000 Lane 3 : 1/10,000 Lane 4 : 1/20,000

文献 (10)

发表研究结果有使用 ab5449？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5449 被引用在 10 文献中.

  • Cui X  et al. Mechanism of TREM2/DAP12 complex affecting ß-amyloid plaque deposition in Alzheimer's disease modeled mice through mediating inflammatory response. Brain Res Bull 166:21-28 (2021). PubMed: 33053435
  • Weavers H  et al. Injury Activates a Dynamic Cytoprotective Network to Confer Stress Resilience and Drive Repair. Curr Biol 29:3851-3862.e4 (2019). PubMed: 31668626
  • Kim MY  et al. Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis. Nucleic Acids Res 46:4933-4949 (2018). PubMed: 29547954
  • Ulrich G  et al. Phosphorylation of nuclear Tau is modulated by distinct cellular pathways. Sci Rep 8:17702 (2018). PubMed: 30531974
  • Boubriak II  et al. Stress-induced release of Oct-1 from the nuclear envelope is mediated by JNK phosphorylation of lamin B1. PLoS One 12:e0177990 (2017). PubMed: 28542436
  • Pabst M  et al. Astrocyte Intermediaries of Septal Cholinergic Modulation in the Hippocampus. Neuron 90:853-65 (2016). PubMed: 27161528
  • Dannenberg H  et al. Synergy of direct and indirect cholinergic septo-hippocampal pathways coordinates firing in hippocampal networks. J Neurosci 35:8394-410 (2015). PubMed: 26041909
  • Weitzel LR  et al. Discovery and verification of protein differences between Er positive/Her2/neu negative breast tumor tissue and matched adjacent normal breast tissue. Breast Cancer Res Treat 124:297-305 (2010). WB . PubMed: 20087651
  • Brown CW  et al. The p14 FAST protein of reptilian reovirus increases vesicular stomatitis virus neuropathogenesis. J Virol 83:552-61 (2009). WB . PubMed: 18971262
  • Luyten A  et al. The PDZ Protein Syntenin Directly Interacts with Frizzled 7 and Supports Non-canonical Wnt Signaling. Mol Biol Cell : (2008). IP . PubMed: 18256285

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Western blot abreview for Anti-GFP antibody

 Good
Abreviews
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - nuclear (Hansenula polymorpha)
Loading amount
10 µg
Specification
Hansenula polymorpha
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Sep 28 2009

Question

I am looking for a polyclonal antibody made by using the same immunogen as this ab6556 but using a different host species.

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Answer

Thank you for your enquiry. I can confirm that ab5450 (affinity purified) and ab5449 (crude) are exactly what you are looking for. These antibodies were made in goat using the exact same antigen and they work just as well as ab6556 in all applications tested.

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Question

I am trying to detect, by western blotting, a chimeric protein expressed in transgenic plants. The chimeric protein consists of green fluorescent protein (GFP) fused to the carboxy terminus of my protein. I am thinking about buying an anti-GFP antibody but I need to be sure that it will not cross-react with cyan fluorescent protein (CFP).

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Answer

Thank you for your enquiry. If your CFP is derived from the GFP of Aequorea victoria jellyfish, then I am afraid all our anti-GFP antibodies will cross-react with that protein, since the difference is a single amino-acid substitution and none of our anti-GFP antibodies are directed against that epitope. If your CFP is from another species, for example the reef coral Anemonia majano, then the antibodies will not cross-react. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

1. What are the concentrations for ab5449. ab5450? 2. What is the difference between ab5449, ab5450, ab1218

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Answer

Thank you for your interesting enquiry. I have had a look at the details for the immunogens on these antibodies and obtained some details for you. ab1218 is a mouse monoclonal which is been raised against a recombinant GFP fusion protein using amino acids 1-246 of GFP from jellyfish Aiquorea Victoria. It is reactive against all varients of this protein. Ab5449 is a goat polyclonal to the full length recombinant GFP protein (from Aiquorea Victoria) prepared in e coli. Its concentration is not available as this antibody is supplied as whole goat antiserum. Ab5450 is the same as Ab5449 but it has been affinipured. This means it is likely to be more specific and stronger binding to the immunogen. Its concentration is 0.5mg/ml.

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Question

Does this antibody recognize EGFP as well?

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Answer

Yes, it is very likely it will detect EGFP as well.

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