Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Calvaria bone regeneration with GFP labeled cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Permeabilization
No
Specification
Calvaria bone regeneration with GFP labeled cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: IgG-HRP Goat Anti-Rabbit Polyclonal Immunoglobulin
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Murine cells expressing GFP ubiquitously are transplanted into bone defects of nude mice. The staining shows the presence of GFP cells involved in bone regeneration.
PubMed ID
10.1016/j.biomaterials.2019.119278: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Queralt Vallmajó
Verified customer
提交于 Mar 19 2020