Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cell line)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
HEK293 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
LiCor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Additional data
Additional Notes
Over-expression of GFP fusion protein (right lane) and empty vector control (left)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 20 2022