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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments

Anti-GFAP抗体(ab7260)

  • Datasheet
  • SDS
Reviews (101)Q&A (10)References (783)

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Immunohistochemistry (PFA fixed) - Anti-GFAP antibody (ab7260)
  • Western blot - Anti-GFAP antibody (ab7260)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
  • Immunoprecipitation - Anti-GFAP antibody (ab7260)
  • Western blot - Anti-GFAP antibody (ab7260)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)

Key features and details

  • Rabbit polyclonal to GFAP
  • Suitable for: IHC (PFA fixed), ICC/IF, IP, WB, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-GFAP antibody [RM1003] (ab278054)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-GFAP抗体
    参阅全部 GFAP 一抗
  • 描述

    兔多克隆抗体to GFAP
  • 宿主

    Rabbit
  • 特异性

    Specifically recognizes mammalian GFAP on western blots and immunocytochemically. Detects a band of 55kDa corresponding to GFAP and also a GFAP derived 48kDa band. Some customers have successfully used ab7260 on Zebrafish lysates; however we have conflicting data to suggest that not all batches will be suitable for work on Zebrafish. For further information, please contact Abcam Scientific Support.
  • 经测试应用

    适用于: IHC (PFA fixed), ICC/IF, IP, WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat
    预测可用于: Horse, Cow, Human, Pig, Mammals
  • 免疫原

    Recombinant full length protein corresponding to Human GFAP. Isotype 1 expressed in and purified from E. coli.

  • 常规说明

    In some cases, the antibody may appear red in color. This is due to small amounts of hemolysis, and does not affect antibody performance.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class II
    • GFAP
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte
    • Neuroscience
    • Development

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GFAP protein (ab114149)
    • Recombinant Human GFAP protein (ab151370)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-GFAP antibody [ZRF-1] (ab154474)
    • Human GFAP Matched Antibody Pair Kit (ab222279)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7260于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC (PFA fixed)
Use at an assay dependent concentration.
ICC/IF (22)
1/5000.
IP
1/30.
WB (13)
1/10000. Detects a band of approximately 55,48 kDa.

This lower 48kDa band is thought to be a degradation product.

IHC-P (26)
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
IHC (PFA fixed)
Use at an assay dependent concentration.
ICC/IF
1/5000.
IP
1/30.
WB
1/10000. Detects a band of approximately 55,48 kDa.

This lower 48kDa band is thought to be a degradation product.

IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性

    Expressed in cells lacking fibronectin.
  • 疾病相关

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性

    Belongs to the intermediate filament family.
  • 翻译后修饰

    Phosphorylated by PKN1.
  • 细胞定位

    Cytoplasm. Associated with intermediate filaments.
  • Target information above from: UniProt accession P14136 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 281189 Cow
    • Entrez Gene: 2670 Human
    • Entrez Gene: 14580 Mouse
    • Entrez Gene: 396562 Pig
    • Entrez Gene: 24387 Rat
    • Omim: 137780 Human
    • SwissProt: Q28115 Cow
    • SwissProt: P14136 Human
    • SwissProt: P03995 Mouse
    • SwissProt: P47819 Rat
    • Unigene: 514227 Human
    • Unigene: 1239 Mouse
    • Unigene: 91512 Rat
    see all
  • 别名

    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

图片

  • Immunohistochemistry (PFA fixed) - Anti-GFAP antibody (ab7260)
    Immunohistochemistry (PFA fixed) - Anti-GFAP antibody (ab7260)

    GFAP antibody ab7260 was used with Tissue Clearing Kit ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain. Blue: DAPI, Green: GFAP.

    Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.

    To use this antibody with tissue clearing, use Tissue Clearing Kit ab243298. For 1 mm brain sections, we recommend a starting dilution of 1:1000, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (ab150077) at a dilution of 1:400.

  • Western blot - Anti-GFAP antibody (ab7260)
    Western blot - Anti-GFAP antibody (ab7260)
    All lanes : Anti-GFAP antibody (ab7260) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat spinal cord lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse spinal cord lysate
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)

    ICC/IF side-by-side comparison with the recombinant multiclonal antibody ab278054
    This ICC/IF image is a comparison between ab7260 and the alternative recombinant multiclonal antibody ab278054.
    Left side - Recombinant multiclonal to GFAP - ab278054
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling GFAP with ab278054 at 1/500 (0.938 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
    Confocal image showing cytoplasmic staining in mouse primary astrocytes. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
    ab10062 Anti-GFAP mouse monoclonal antibody at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red) to counterstain. The nuclear counterstain was DAPI (Blue).
    Negative control 1: ab278054 at a 1/500 dilution followed by ab150120 at a 1/500 dilution.
    Negative control 2: ab10062 at a 1/500 dilution followed by ab150077 at a 1/1000 dilution.
    Right side - Polyclonal antibody to [GFAP] - ab7260
    Same testing conditions as ab278054.
    Why choose a recombinant antibody?
    Research with confidence - consistent and reproducible results with every batch
    Long-term and scalable supply - powered by recombinant technology for fast production
    Success from the first experiment - confirmed specificity through extensive validation
    Ethical standards compliant - production is animal-free

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)

    IHC-P side-by-side comparison with the recombinant multiclonal antibody ab278054
    This IHC-P image is a comparison between ab7260 and the alternative recombinant multiclonal antibody ab278054.
    Left side - Recombinant multiclonal to GFAP - ab278054
    IHC image of GFAP staining in a formalin fixed, paraffin embedded normal rat brain tissue section, performed on a Leica Bond™ system using the standard protocol F.
    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab278054 at 1/2000 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
    Right side - Polyclonal antibody to [GFAP] - ab7260
    Same testing conditions as ab278054.
    Why choose a recombinant antibody?
    Research with confidence - consistent and reproducible results with every batch
    Long-term and scalable supply - powered by recombinant technology for fast production
    Success from the first experiment - confirmed specificity through extensive validation
    Ethical standards compliant - production is animal-free

  • Immunoprecipitation - Anti-GFAP antibody (ab7260)
    Immunoprecipitation - Anti-GFAP antibody (ab7260)

    Immunoprecipitation side-by-side comparison with the recombinant multiclonal antibody ab278054
    This immunoprecipitation image is a comparison between ab7260 and the alternative recombinant multiclonal antibody ab278054.
    Left side - Recombinant multiclonal to GFAP - ab278054
    GFAP was immunoprecipitated from 0.35 mg mouse brain lysate with ab278054 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278054 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
    Lane 1: Mouse brain lysate 10 µg.
    Lane 2: ab278054 IP in mouse brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278054 in mouse brain lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.
    Right side - Polyclonal antibody to [GFAP] - ab7260
    Same testing conditions as ab278054.
    Why choose a recombinant antibody?
    Research with confidence - consistent and reproducible results with every batch
    Long-term and scalable supply - powered by recombinant technology for fast production
    Success from the first experiment - confirmed specificity through extensive validation
    Ethical standards compliant - production is animal-free

  • Western blot - Anti-GFAP antibody (ab7260)
    Western blot - Anti-GFAP antibody (ab7260)This image is courtesy of an anonymous Abreview
    All lanes : Anti-GFAP antibody (ab7260) at 1/5000 dilution

    Lanes 1-3 : Rat thoracotomy, spinal cord homogenate
    Lanes 4-5 : Rat thoracotomy sham, spinal cord homogenate
    Lanes 6-7 : Rat nerve transect sham, spinal cord homogenate
    Lanes 8-9 : Rat nerve transect, spinal cord homogenate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit at 1/3000 dilution

    Developed using the ECL technique.

    Observed band size: 53 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)

    IHC image of GFAP staining in a formalin fixed, paraffin embedded normal mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol B.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7260 at 1/2000 dilution for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)Yoon, H. et al PLoS One. 2017 Jul 10;12(7):e0180697. doi: 10.1371/journal.pone.0180697. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Increases in GFAP after demyelinating injury are greater in the spinal cord compared to brain

    Photomicrographs show immunoreactivity for GFAP or ALDH1L1 in (A) the corpus callosum, or (B) the dorsal column white matter of adult mice at base line, and at 14 d after microinjection of the demyelinating agent lysolecithin. Histograms show the percent area of GFAP immunofluorescence, and expression of GFAP/ALDH1L1+ astrocyte, was significantly greater in the spinal cord compared to the corpus callosum 14d post-lysolecithin lesion.

    GFAP was detected using ab7260.

    (From Figure 4A and 4B of Hoon et al)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)

    IHC image of GFAP staining in a formalin fixed, paraffin embedded normal rat brain tissue section, performed on a Leica Bond™ system using the standard protocol F.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7260 at 1/2000 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)This image is courtesy of an Abreview submitted by Chia-Li Lin.

    ab7260 staining GFAP in cells from mouse brain tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 in PBS and blocked with 1% BSA for 40 minutes at 25°C. Samples were incubated with primary antibody (1/1200 in TBS) for 24 hours at 4°C. Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883) was used as the secondary antibody at a dilution of 1/200.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)

    ab7260 at 1/10000 dilution staining mouse cortical astrocytes by Immunocytochemistry.

    The cells were permeabilized with Triton/HEPES buffer prior to primary application. The antibody was incubated with the cells for 18 hours and then bound antibody was detected with an Alexa Fluor ® 488 conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submited by Charmaine Noonan.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)This image is courtesy of an Abreview submitted by Mr Osama Mohsen

    ab7260 staining rat brain tissue sections by IHC-P.

    Sections were fixed in formaldehyde and blocked with a commercialy available blocking agent prior to incubating with ab7260, diluted 1/5000 for 20 hours at 4°C.  An HRP conjugated mouse polyclonal (universal HRP polymer detection) antibody was used as the secondary.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy

     ab7260 staining rat pup cortical preps by ICC/IF.

    The preps were grown for 14 days in culture and plated onto coverslips. The preps were acid/alcohol fixed and blocked prior to incubation with ab7260. Bound antibody was detected using an Alexa Fluor ®488 conjugated goat polyclonal antibody. Nuclei were visualized using DAPI.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody (ab7260)This image was kindly supplied by Dr Vladimir Milenkovic by Abreview

    ab7260 staining GFAP in mouse eye tissue sections by Immunohistochemistry (paraffin embedded sections).

    Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C; followed by incubation with the primary antibody, at a 1/500 dilution, for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 used at a 1/5000 dilution.

    The retinal layers are: ganglion cells layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), and photoreceptor outer segments (ROS). Nuclei were counterstained with DAPI.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody (ab7260)

    GFAP antibody ab7260 was used with 3D Cell Culture Clearing Kit ab243299 to penetrate, stain and clear a 3D neuronal spheroid cell culture. Blue: DAPI, Green: GFAP.

    Learn more about 3D cell culture clearing and tissue clearing kits, reagents, and protocols designed to make it easier to stain 3D cell cultures and thick tissue sections and get more data from each valuable tissue section. 

    To use this antibody with clearing, use our 3D Cell Culture Clearing Kit ab243299. We recommend a starting dilution of 1:1000, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (ab150077) at a dilution of 1:400.

实验方案

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (783)

发表研究结果有使用 ab7260?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7260 被引用在 783 文献中.

  • Qu X  et al. Levistolide A Attenuates Alzheimer's Pathology Through Activation of the PPAR? Pathway. Neurotherapeutics 18:326-339 (2021). PubMed: 33034847
  • Sinha P  et al. Trehalose Ameliorates Seizure Susceptibility in Lafora Disease Mouse Models by Suppressing Neuroinflammation and Endoplasmic Reticulum Stress. Mol Neurobiol 58:1088-1101 (2021). PubMed: 33094475
  • Bai G  et al. LncRNA Neat1 Promotes Regeneration after Spinal Cord Injury by Targeting miR-29b. J Mol Neurosci 71:1174-1184 (2021). PubMed: 33175349
  • Mansor NI  et al. Asymptomatic neurotoxicity of amyloid ß-peptides (Aß1-42 and Aß25-35) on mouse embryonic stem cell-derived neural cells. Bosn J Basic Med Sci 21:98-110 (2021). PubMed: 32156249
  • Endicott SJ  et al. Long-lived mice with reduced growth hormone signaling have a constitutive upregulation of hepatic chaperone-mediated autophagy. Autophagy 17:612-625 (2021). PubMed: 32013718
View all Publications for this product

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Immunohistochemistry (Frozen sections) abreview for Anti-GFAP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain (cortex))
Permeabilization
No
Specification
Brain (cortex)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 27 2022

Immunohistochemistry (Frozen sections) abreview for Anti-GFAP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain (cortex))
Permeabilization
No
Specification
Brain (cortex)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Acetone
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Jan 27 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic intestinal cells)
Permeabilization
Yes - TritonX100 0.5%
Specification
embryonic intestinal cells
Blocking step
PBSNaN3 Horse serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 26 2021

Western blot abreview for Anti-GFAP antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain Lysates)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Brain Lysates
Blocking step
commercial blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 May 03 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (10 micon thick spinal cord sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vector Laboratories Cat# H-3300
Permeabilization
Yes - 0.5% Triton
Specification
10 micon thick spinal cord sections
Blocking step
normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 09 2020

Immunohistochemistry (Frozen sections) abreview for Anti-GFAP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.05% triton-x100
Specification
Brain
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 07 2019

Western blot abreview for Anti-GFAP antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Brain
Blocking step
Protein Free blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 18 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 09 2019

Immunohistochemistry free floating abreview for Anti-GFAP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Adult rat brainstem tissue)
Specification
Adult rat brainstem tissue
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 28 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Brain cortex
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 20 2018

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