概述

  • 产品名称
  • 描述
    兔多克隆抗体to GFAP
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-Fr, ICC, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Guinea pig, Hamster, Cow, Cat, Human, Kangaroo, Monkey
  • 免疫原

    Full length native protein (purified) corresponding to Cow GFAP. Isolated from cow spinal cord.

  • 阳性对照
    • ICC/IF: Mouse cortical primary astrocytes. IHC-P: Human brain tissue.
  • 常规说明


    In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.

性能

应用

Our Abpromise guarantee covers the use of ab16997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 24223835
ICC 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 20460993
IHC-P 1/50 - 1/250.

靶标

  • 功能
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性
    Expressed in cells lacking fibronectin.
  • 疾病相关
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylated by PKN1.
  • 细胞定位
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

图片

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue, staining GFAP with ab16997.

  • Mouse cortical primary astrocytes stained for GFAP using ab16997 at a dilution of 1/100 in ICC/IF.

  • Formalin-fixed, paraffin-embedded section of brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.

文献

This product has been referenced in:
  • Sanderson TH  et al. Inhibitory modulation of cytochrome c oxidase activity with specific near-infrared light wavelengths attenuates brain ischemia/reperfusion injury. Sci Rep 8:3481 (2018). Read more (PubMed: 29472564) »
  • Kuwahara H  et al. Modulation of blood-brain barrier function by a heteroduplex oligonucleotide in vivo. Sci Rep 8:4377 (2018). IHC . Read more (PubMed: 29531265) »
See all 24 Publications for this product

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Answer

After completing a short literature search, GFAP is commonly used as an protoplasmic astrocyte marker. Below are two references:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0038243
http://www.jneurosci.org/content/22/1/183.full.pdf

We stock 30 antibodies that are tested in IHC on human cells.

Read More

Answer

I can confirm that ab16997 will only detect GFAP in astorcytes not in oligodendrocytes and micogliocytes (these cells do not express this protein).

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
None
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C

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Verified customer

提交于 Aug 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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