Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 24°C
Other product details
Dilution
1/2000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 1% Triton X-100 + 0.2% milk
Secondary antibody
Name
Non-Abcam antibody was used: Sheep anti-mouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Biotin
Host species: Sheep
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400
Additional data
Additional Notes
Fixative concentration 4% PFA. Endogenous peroxidase blocking by 0,3% H2O2 in MetOH for 20 minutes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Marian Hruska-Plochan
Verified customer
提交于 Feb 23 2010