Anti-GFAP抗体[2A5] (ab4648)
Key features and details
- Mouse monoclonal [2A5] to GFAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-GFAP抗体[2A5]
参阅全部 GFAP 一抗 -
描述
小鼠单克隆抗体[2A5] to GFAP -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig -
免疫原
Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.
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阳性对照
- IHC-P: Human brain tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.
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常规说明
This clone gives a much stronger signal in pig, cow and human samples than in rodents. For rodent samples use ab68428.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
Concentrated Tissue Culture Supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant. -
克隆
单克隆 -
克隆编号
2A5 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
Our Abpromise guarantee covers the use of ab4648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | 1/2000. Predicted molecular weight: 49 kDa. | |
IHC-P | 1/2000. | |
ICC/IF | 1/10 - 1/50. |
靶标
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功能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
组织特异性
Expressed in cells lacking fibronectin. -
疾病相关
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylated by PKN1. -
细胞定位
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 396562 Pig
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
see all -
别名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
图片
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Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.
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Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution
Lane 1 : MW markers
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Lane 6 : Pig brain tissue lysate
Lane 7 : Recombinant rat GFAP protein
Lane 8 : Recombinant human GFAP protein
Predicted band size: 49 kDa -
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
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Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
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Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).
文献 (45)
ab4648 被引用在 45 文献中.
- Yang X et al. SMART-Q: An Integrative Pipeline Quantifying Cell Type-Specific RNA Transcription. PLoS One 15:e0228760 (2020). PubMed: 32348304
- Dobrachinski F et al. Guanosine Attenuates Behavioral Deficits After Traumatic Brain Injury by Modulation of Adenosinergic Receptors. Mol Neurobiol 56:3145-3158 (2019). PubMed: 30105669
- You Y et al. Demyelination precedes axonal loss in the transneuronal spread of human neurodegenerative disease. Brain 142:426-442 (2019). PubMed: 30668642
- Zhou F et al. HIV-1 Tat enhances purinergic P2Y4 receptor signaling to mediate inflammatory cytokine production and neuronal damage via PI3K/Akt and ERK MAPK pathways. J Neuroinflammation 16:71 (2019). PubMed: 30947729
- Xu P et al. Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke. Cell Death Dis 10:555 (2019). PubMed: 31324751