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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments

Anti-GFAP抗体[2A5] (ab4648)

  • Datasheet
  • SDS
Reviews (6)Q&A (3)References (45)

Product price, shipping and contact information

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Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)
  • Western blot - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

Key features and details

  • Mouse monoclonal [2A5] to GFAP
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-GFAP抗体[2A5]
    参阅全部 GFAP 一抗
  • 描述

    小鼠单克隆抗体[2A5] to GFAP
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.

  • 阳性对照

    • IHC-P: Human brain tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.
  • 常规说明

    This clone gives a much stronger signal in pig, cow and human samples than in rodents. For rodent samples use ab68428.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.065% Sodium azide
    Constituent: Tissue culture supernatant

    Concentrated Tissue Culture Supernatant
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 纯化说明

    Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant.
  • 克隆

    单克隆
  • 克隆编号

    2A5
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class II
    • GFAP
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human GFAP protein (ab114149)
    • Recombinant Human GFAP protein (ab151370)
  • Related Products

    • Human GFAP Matched Antibody Pair Kit (ab222279)

应用

Our Abpromise guarantee covers the use of ab4648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 49 kDa.
IHC-P 1/2000.
ICC/IF 1/10 - 1/50.

靶标

  • 功能

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • 组织特异性

    Expressed in cells lacking fibronectin.
  • 疾病相关

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • 序列相似性

    Belongs to the intermediate filament family.
  • 翻译后修饰

    Phosphorylated by PKN1.
  • 细胞定位

    Cytoplasm. Associated with intermediate filaments.
  • Target information above from: UniProt accession P14136 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2670 Human
    • Entrez Gene: 14580 Mouse
    • Entrez Gene: 396562 Pig
    • Entrez Gene: 24387 Rat
    • Omim: 137780 Human
    • SwissProt: P14136 Human
    • SwissProt: P03995 Mouse
    • SwissProt: P47819 Rat
    • Unigene: 514227 Human
    • Unigene: 1239 Mouse
    • Unigene: 91512 Rat
    see all
  • 别名

    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

图片

  • Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry - Free Floating - Anti-GFAP antibody [2A5] (ab4648)

    Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.

     

  • Western blot - Anti-GFAP antibody [2A5] (ab4648)
    Western blot - Anti-GFAP antibody [2A5] (ab4648)
    Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution

    Lane 1 : MW markers
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat spinal cord tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse spinal cord tissue lysate
    Lane 6 : Pig brain tissue lysate
    Lane 7 : Recombinant rat GFAP protein
    Lane 8 : Recombinant human GFAP protein

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)

    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
    Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [2A5] (ab4648)
    Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).

实验方案

  • Recommended ICC protocol with ab4648
  • Protocol Booklet

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (45)

    发表研究结果有使用 ab4648?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4648 被引用在 45 文献中.

    • Yang X  et al. SMART-Q:  An Integrative Pipeline Quantifying Cell Type-Specific RNA Transcription. PLoS One 15:e0228760 (2020). PubMed: 32348304
    • Dobrachinski F  et al. Guanosine Attenuates Behavioral Deficits After Traumatic Brain Injury by Modulation of Adenosinergic Receptors. Mol Neurobiol 56:3145-3158 (2019). PubMed: 30105669
    • You Y  et al. Demyelination precedes axonal loss in the transneuronal spread of human neurodegenerative disease. Brain 142:426-442 (2019). PubMed: 30668642
    • Zhou F  et al. HIV-1 Tat enhances purinergic P2Y4 receptor signaling to mediate inflammatory cytokine production and neuronal damage via PI3K/Akt and ERK MAPK pathways. J Neuroinflammation 16:71 (2019). PubMed: 30947729
    • Xu P  et al. Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke. Cell Death Dis 10:555 (2019). PubMed: 31324751
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-9 of 9 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody [2A5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain tissue section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Brain tissue section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 28 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GFAP antibody [2A5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization
    Yes - 0.1% Tween 20 in PBS
    Specification
    spinal cord
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Chia-Li Lin

    Verified customer

    提交于 Nov 24 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody [2A5]

    Poor
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RTP°C
    Sample
    Mouse Cell (Mouse primary astrocyte)
    Specification
    Mouse primary astrocyte
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Dr. Craig Beall

    Verified customer

    提交于 Sep 15 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-GFAP antibody [2A5] - Astrocyte Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 08 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GFAP antibody [2A5] - Astrocyte Marker

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Embryonic rat cerebral cortex)
    Specification
    Embryonic rat cerebral cortex
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Ice cold methanol
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 26 2012

    Question

    Application: sELISA
    Capture ab: ab4648, ELISA coating buffer, ph 9.9, polystyrene plate, 0.2 ug/mL O/N @ 4oC
    Wash twice with PBS
    Block (PBS + 5% goat serum) 90 min. @ RT. Tried 3% BSA as well
    Introduce antigen: 2 hr. RT up to 100 ng/mL (within detection range on ds)
    Detection ab: 48050. 2 hrs. @ RT, 10ug/mL in 5% goat serum
    Wash 3 times PBS
    Add secondary: goat anti-rabbit IgG, streptavidin-gold conjugated
    Sample: human GFAP
    Problem: No signal detected

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2012

    Answer

    Thank you for contacting Abcam.

    Earlier we discussed your sELISA with ab4648 as the capture and ab48050 as the detector. As we discussed, since you would like to use your streptavidin-gold conjugated secondary, a detector that is conjugated to biotin is preferable so you can obtain the signal amplification necessary to have a detectable signal. Thus, we discussed testing ab79203 as your detector antibody through our AbTrial program. Your discount code and information about the abtrial program is given below:

    I am very pleased to hear you would like to accept our offer and test ab79203 in sELISA. This code will give you $XXX off your next order before the expiration date. To redeem this offer, please submit an Abreview for sELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial

    On the phone I forgot to mention our current RabMab promotion, which entitles you to a free rabbit monoclonal antibody with any purchase of a primary antibody. Quote “RABMAB-XBSMG” in your next primary antibody order to take advantage of this offer. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Nov 30 2012

    Question

    Can you please tell me the protocol used to validate ab4648 and ab48050 as a sandwich ELISA pair?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 20 2012

    Answer

    The protocol used was as follows:
    Buffers and Reagents:
    Carbonate Coating Buffer (100 mM)
    Antigen or antibody should be diluted in coating buffer to immobilize them to the wells:
    3.03 g Na2CO3,
    6.0 g NaHCO3
    Make up to 1000 ml with distilled water and adjust to pH 9.6
    PBS:
    1.16 g Na2HPO4,
    0.1 g KCl,
    0.1 g K3PO4
    4.0 g NaCl
    Make up to 500 ml with distilled water and adjust to pH 7.4
    Wash Solution - PBST (0.1% (v/v) Tween):
    Add 5ml 10% (v/v) Tween-20 stock solution to 500ml PBS,
    Blocking and Antibody/Protein Dilution Buffer:
    5% (w/v) BSA in PBST.
    Stop Solution:
    0.25M Sulphuric Acid
    Protocol:
    1. 96-well ELISA plates are coated with 100µl/well of Capture Antibody ab4648 diluted 1:5000 in 100mM Carbonate Coating buffer, pH9.6.
    2. Incubate the plates overnight at 4°C or 1 hour at room temperature to allow adsorption of the Capture Antibody to the plate.
    3. Remove the Capture Antibody and was the plate 3 times with PBST (3x300µl/well), leaving the plate dry after the final wash.
    4. Block the plates with 150µl/well of Blocking Buffer.
    5. Incubate plates at room temperature for 1 hour.
    6. Aspirate Blocking Buffer and add 50µl/well of Target Protein at the recommended concentration in Blocking Buffer.
    7. Incubate for 1 hour at room temperature.
    8. Remove the Protein Solution and was the plate 3 times with PBST (300µl/well), leaving the plate dry after the final wash.
    9. Add 50µl/well of Detector Antibody ab48050 diluted to 0.5 ug/mL in Blocking Buffer.
    10. Incubate for 1 hour at room temperature.
    11. Remove the Detector Antibody solution and wash the plate 3 times with PBST (300µl/well), leaving the plate dry after the final wash.
    12. Aliquot 100µl/well of anti-rabbit HRP conjugate Secondary Antibody diluted 1:10,000 in Blocking Buffer.
    13. Incubate for 1 hour at room temperature.
    14. Remove the Detector Antibody solution and was the plate 3 times with PBST (300µl/well), followed by 2 washes with PBS, leaving the plate dry after the final wash.
    15. Add 50µl/well of TMB Peroxidase Substrate.
    16. Incubate for 10 minutes at room temperature (The length of this incubation may be optimised, but 10 minutes works well in most cases).
    17. Add 50µl/well of 0.25M Sulphuric Acid Stop Solution.
    18. Read plates on plate reader at 450nm.

    Read More

    Abcam Scientific Support

    回复于 Jul 20 2012

    Question

    Is antigen retrieval recommended with this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2012

    Answer

    We do recommend usingthe Sodium Citrate buffer (pH 6.0), however HIER are effective as well.

    Read More

    Abcam Scientific Support

    回复于 Feb 09 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-GFAP antibody [2A5] - Astrocyte Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 24°C
    Read More

    Mr. Marian Hruska-Plochan

    Verified customer

    提交于 Feb 23 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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