Key features and details
- Mouse monoclonal [2A5] to GFAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG1
参阅全部 GFAP 一抗
描述小鼠单克隆抗体[2A5] to GFAP
经测试应用适用于: WB, IHC-P, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Human, Pig
Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.
- IHC-P: Human brain tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.
This clone gives a much stronger signal in pig, cow and human samples than in rodents. For rodent samples use ab68428.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
Concentrated Tissue Culture Supernatant
Concentration information loading...
纯度Tissue culture supernatant
纯化说明Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant.
Our Abpromise guarantee covers the use of ab4648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 49 kDa.|
|ICC/IF||1/10 - 1/50.|
功能GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
组织特异性Expressed in cells lacking fibronectin.
疾病相关Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
序列相似性Belongs to the intermediate filament family.
翻译后修饰Phosphorylated by PKN1.
细胞定位Cytoplasm. Associated with intermediate filaments.
- Information by UniProt
- wu:fb34h11 antibody
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Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.
Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution
Lane 1 : MW markers
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Lane 6 : Pig brain tissue lysate
Lane 7 : Recombinant rat GFAP protein
Lane 8 : Recombinant human GFAP protein
Predicted band size: 49 kDa
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).
Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).
ab4648 被引用在 45 文献中.
- Yang X et al. SMART-Q: An Integrative Pipeline Quantifying Cell Type-Specific RNA Transcription. PLoS One 15:e0228760 (2020). PubMed: 32348304
- Dobrachinski F et al. Guanosine Attenuates Behavioral Deficits After Traumatic Brain Injury by Modulation of Adenosinergic Receptors. Mol Neurobiol 56:3145-3158 (2019). PubMed: 30105669
- You Y et al. Demyelination precedes axonal loss in the transneuronal spread of human neurodegenerative disease. Brain 142:426-442 (2019). PubMed: 30668642
- Zhou F et al. HIV-1 Tat enhances purinergic P2Y4 receptor signaling to mediate inflammatory cytokine production and neuronal damage via PI3K/Akt and ERK MAPK pathways. J Neuroinflammation 16:71 (2019). PubMed: 30947729
- Xu P et al. Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke. Cell Death Dis 10:555 (2019). PubMed: 31324751