参阅全部 Gephyrin 一抗
描述兔单克隆抗体[EPR12650] to Gephyrin
经测试应用适用于: ICC/IF, IHC-P, WBmore details
种属反应性与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Dog, Pig, Zebrafish, Rhesus monkey, Xenopus tropicalis
Synthetic peptide within Human Gephyrin aa 1-100. The exact sequence is proprietary.
Database link: Q9NQX3
- Fetal brain tissue lysate, 293T and SH-SY5Y cell lysates; Human brain tissue; 293 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab181382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 93 kDa (predicted molecular weight: 80 kDa).|
功能Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
通路Cofactor biosynthesis; molybdopterin biosynthesis.
疾病相关Defects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
序列相似性In the N-terminal section; belongs to the moaB/mog family.
In the C-terminal section; belongs to the moeA family.
细胞定位Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes.
- Information by UniProt
- Domain E antibody
- Domain G antibody
- GEPH antibody
All lanes : Anti-Gephyrin antibody [EPR12650] (ab181382) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GPHN (Gephyrin) knockout HAP1 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : U2OS whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 80 kDa
Lanes 1 - 4: Merged signal (red and green). Green - ab181382 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab181382 was shown to recognize Gephyrin in wild-type HAP1 cells as signal was lost at the expected MW in GPHN (Gephyrin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPHN (Gephyrin) knockout samples were subjected to SDS-PAGE. Ab181382 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent staining of 293 cells using anti-Gephyrin RabMAb (red) DAPI nuclear staining (blue)
Immunohistochemical analysis of parafiin embedded human brain tissue using anti-Gephyrin RabMAb
All lanes : Anti-Gephyrin antibody [EPR12650] (ab181382) at 1/1000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 80 kDa
ab181382 has not yet been referenced specifically in any publications.