概述

  • 产品名称
    Anti-Geminin抗体
    参阅全部 Geminin 一抗
  • 描述
    兔多克隆抗体to Geminin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Geminin aa 1-209.
    Sequence:

    MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSK RKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWK EVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQY MAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVS SSTDAKPCI


    Database link: O75496

  • 阳性对照
    • SW480, 22RV-1, HL60, HepG2, MCF7 and K562 cell line extracts.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab187553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 24 kDa.

靶标

  • 功能
    Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
  • 序列相似性
    Belongs to the geminin family.
  • 发展阶段
    Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA replication inhibitor antibody
    • Gem antibody
    • GEMI_HUMAN antibody
    • Geminin antibody
    • Geminin DNA replication inhibitor antibody
    • GMNN antibody
    • OTTHUMP00000039393 antibody
    • RP3 369A17.3 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling Geminin with ab187553 at 1/200. Magnification: 200x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Geminin with ab187553 at 1/200. Magnification: 200x.
  • All lanes : Anti-HIF1AN antibody (ab187524) at 1/500 dilution

    Lane 1 : SW480 cell line extract
    Lane 2 : 22RV-1 cell line extract
    Lane 3 : HL60 cell line extract
    Lane 4 : HepG2 cell line extract
    Lane 5 : MCF7 cell line extract
    Lane 6 : K562 cell line extract

    Predicted band size: 24 kDa

文献

ab187553 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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