Anti-Gemin 2抗体[2E17] (ab6084)
Key features and details
- Mouse monoclonal [2E17] to Gemin 2
- Suitable for: Flow Cyt
- Reacts with: Human, Xenopus laevis
- Isotype: IgG1
概述
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产品名称
Anti-Gemin 2抗体[2E17]
参阅全部 Gemin 2 一抗 -
描述
小鼠单克隆抗体[2E17] to Gemin 2 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human, Xenopus laevis -
免疫原
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).
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常规说明
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from supernatant. -
Primary antibody说明
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5. -
克隆
单克隆 -
克隆编号
2E17 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab6084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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靶标
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功能
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. -
序列相似性
Belongs to the gemin-2 family. -
细胞定位
Nucleus > gem. Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8487 Human
- Omim: 602595 Human
- SwissProt: O14893 Human
- SwissProt: O42260 Xenopus laevis
- Unigene: 652307 Human
- Unigene: 3305 Xenopus laevis
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别名
- Component of gems 2 antibody
- Gem (nuclear organelle) associated protein 2 antibody
- Gem associated protein 2 antibody
see all
图片
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![Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.cn/ps/products/6/ab6084/Images/ab6084-12015-anti-gemin-2-antibody-2e17-flow-cytometry.jpg)
Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunocytochemistry/ Immunofluorescence - Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.cn/ps/products/6/ab6084/Images/ab6084-12014-anti-gemin-2-antibody-2e17-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 2 antibody [2E17] (ab6084)Image from Morency E et al, J Cell Biol. 2007 Jun 4;177(5):757-68, Fig 4f. DOI 10.1083/jcb.200612107.HeLa cells were transfected with an ICP0-expressing plasmid before IF assays to detect the distribution of CB- and gem-associated proteins. ab6084 used at a 1/200 dilution to detect Gemin 2 (green). Bar 5 µm.
实验方案
文献 (8)
ab6084 被引用在 8 文献中.
- Staropoli JF et al. Rescue of gene-expression changes in an induced mouse model of spinal muscular atrophy by an antisense oligonucleotide that promotes inclusion of SMN2 exon 7. Genomics 105:220-8 (2015). PubMed: 25645699
- Groen EJ et al. ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN. Hum Mol Genet N/A:N/A (2013). PubMed: 23681068
- Sanchez G et al. A novel function for the survival motoneuron protein as a translational regulator. Hum Mol Genet : (2012). PubMed: 23136128
- Wu CY et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). WB ; Mouse . PubMed: 21385431
- Burnett BG et al. Regulation of SMN protein stability. Mol Cell Biol 29:1107-15 (2009). WB ; Human . PubMed: 19103745
- Walker MP et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; Mouse . PubMed: 18689355
- Morency E et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; Human . PubMed: 17548509
- Renvoisé B et al. Distinct domains of the spinal muscular atrophy protein SMN are required for targeting to Cajal bodies in mammalian cells. J Cell Sci 119:680-92 (2006). PubMed: 16449324
