Anti-GCSF Receptor抗体(ab126167)
Key features and details
- Rabbit polyclonal to GCSF Receptor
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GCSF Receptor抗体
参阅全部 GCSF Receptor 一抗 -
描述
兔多克隆抗体to GCSF Receptor -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within internal sequence amino acids 164-392 of Human GCSF Receptor (UniProt Q99062).
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阳性对照
- IHC-P: Human breast carcinoma tissue. IF: THP-1 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126167于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 92 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 92 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
靶标
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功能
Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. -
组织特异性
One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta. -
疾病相关
Hereditary neutrophilia -
序列相似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1441 Human
- Omim: 138971 Human
- SwissProt: Q99062 Human
- Unigene: 524517 Human
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别名
- CD 114 antibody
- CD114 antibody
- CD114 antigen antibody
see all
图片
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Formalin-fixed, paraffin embedded human breast carcinoma tissue stained fro GCSF receptor usinf a126167 at 1/250 dilution in immunohistochemical analysis,
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min
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Immunofluorescence analysis of THP-1 (human monocytic leukemia cell line) cells labeling GCSF (green) using ab126167 at 1/500 dilution. Blue: Hoechst 33342 staining. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
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All lanes : Anti-GCSF Receptor antibody (ab126167) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 92 kDa7.5% SDS-PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab126167 被引用在 6 文献中.
- Zhang JY et al. Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling via Induction of G-CSF. Front Cell Dev Biol 8:601043 (2020). PubMed: 33415106
- Magistroni V et al. De novo UBE2A mutations are recurrently acquired during chronic myeloid leukemia progression and interfere with myeloid differentiation pathways. Haematologica 104:1789-1797 (2019). PubMed: 30819912
- Dwivedi P et al. Phospho serine and threonine analysis of normal and mutated granulocyte colony stimulating factor receptors. Sci Data 6:21 (2019). PubMed: 30967555
- Zhang H et al. Unpaired Extracellular Cysteine Mutations of CSF3R Mediate Gain or Loss of Function. Cancer Res 77:4258-4267 (2017). PubMed: 28652245
- Maxson JE et al. The Colony-Stimulating Factor 3 Receptor T640N Mutation Is Oncogenic, Sensitive to JAK Inhibition, and Mimics T618I. Clin Cancer Res 22:757-64 (2016). WB ; Human . PubMed: 26475333
- Maxson JE et al. Ligand independence of the T618I mutation in the colony-stimulating factor 3 receptor (CSF3R) protein results from loss of O-linked glycosylation and increased receptor dimerization. J Biol Chem 289:5820-7 (2014). WB ; Human . PubMed: 24403076