Anti-GCP6抗体(ab95172)
Key features and details
- Rabbit polyclonal to GCP6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GCP6抗体 -
描述
兔多克隆抗体to GCP6 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Orangutan -
免疫原
Synthetic peptide corresponding to Human GCP6 aa 1350-1450.
Database link: Q96RT7 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95172于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 200 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 200 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
The protein encoded by this gene is part of a large multisubunit complex required for microtubule nucleation at the centrosome. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 85378 Human
- Omim: 610053 Human
- SwissProt: Q96RT7 Human
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别名
- Gamma tubulin complex component 6 antibody
- GCP 6 antibody
- MCCRP antibody
see all
图片
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All lanes : Anti-GCP6 antibody (ab95172) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 200 kDa -
Lane 1: 3µg ab95172 were used to immunoprecipitate GCP3 from 1mg HeLa whole cell lysate. 20% IP was loaded per lane and probed with ab95170 at 1µg/ml.
Lane 2: same, using a control IgG.
Detection: chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab95172 被引用在 6 文献中.
- Shankar S et al. Α γ-tubulin complex-dependent pathway suppresses ciliogenesis by promoting cilia disassembly. Cell Rep 41:111642 (2022). PubMed: 36384111
- Lindström L & Alvarado-Kristensson M Characterization of gamma-tubulin filaments in mammalian cells. Biochim Biophys Acta 1865:158-171 (2018). WB . PubMed: 29050966
- Cota RR et al. MZT1 regulates microtubule nucleation by linking ?TuRC assembly to adapter-mediated targeting and activation. J Cell Sci 130:406-419 (2017). PubMed: 27852835
- Farache D et al. Functional Analysis of ?-Tubulin Complex Proteins Indicates Specific Lateral Association via Their N-terminal Domains. J Biol Chem 291:23112-23125 (2016). PubMed: 27660388
- Lecland N & Lüders J The dynamics of microtubule minus ends in the human mitotic spindle. Nat Cell Biol 16:770-8 (2014). PubMed: 24976384
- Singh P et al. TACC3 protein regulates microtubule nucleation by affecting ?-tubulin ring complexes. J Biol Chem 289:31719-35 (2014). PubMed: 25246530