Anti-GCLM抗体(ab154017)
Key features and details
- Rabbit polyclonal to GCLM
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GCLM抗体
参阅全部 GCLM 一抗 -
描述
兔多克隆抗体to GCLM -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide, corresponding to a region within amino acids 29-120 of Human GCLM (Uniprot ID: P48507).
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阳性对照
- H1299, 293T, A431, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human BT474 xenograft tissue; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154017于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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组织特异性
In all tissues examined. Highest levels in skeletal muscle. -
通路
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. -
序列相似性
Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily. - Information by UniProt
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数据库链接
- Entrez Gene: 525659 Cow
- Entrez Gene: 2730 Human
- Entrez Gene: 14630 Mouse
- Entrez Gene: 29739 Rat
- Omim: 601176 Human
- SwissProt: Q2T9Y6 Cow
- SwissProt: P48507 Human
- SwissProt: O09172 Mouse
see all -
别名
- Gamma ECS regulatory subunit antibody
- Gamma-ECS regulatory subunit antibody
- Gamma-glutamylcysteine synthetase regulatory subunit antibody
see all
图片
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Anti-GCLM antibody (ab154017) at 1/5000 dilution + HT1299 whole cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue labeling GCML with ab154017 at 1/500 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GCLM with ab154017 at 1/200 dilution. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab154017 被引用在 1 文献中.
- Monti P et al. Mutant p53K120R expression enables a partial capacity to modulate metabolism. Front Genet 13:974662 (2022). PubMed: 36226181