重组Anti-GBP1抗体[1B1] - BSA and Azide free (ab255788)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1B1] to GBP1 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GBP1抗体[1B1] - BSA and Azide free
参阅全部 GBP1 一抗 -
描述
大鼠单克隆抗体[1B1] to GBP1 - BSA and Azide free -
宿主
Rat -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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阳性对照
- WB: HeLa treated with 100 U/ml IFN gamma (ab9659) for 24 hours, whole cell lysate. IHC-P: Human spleen and tonsil tissue. ICC/IF: HeLa treated with IFN gamma (100 U/ml 24h).
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常规说明
ab255788 is the carrier-free version of ab252832.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1B1 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab255788于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Binds GTP, GDP and GMP. -
序列相似性
Belongs to the GBP family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2633 Human
- Omim: 600411 Human
- SwissProt: P32455 Human
- Unigene: 62661 Human
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别名
- GBP 1 antibody
- GBP-1 antibody
- GBP1 antibody
see all
图片
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All lanes : Anti-GBP1 antibody [1B1] (ab252832) at 1/1000 dilution
Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa treated with 100U/ml IFN gamma (ab9659) for 24 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 68 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsThe expression profile observed is consistent with what has been described in the literature (PMID:19079332)
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252832).
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Formalin-fixed, paraffin-embedded human spleen tissue stained for GBP1 using ab252832 at 1/100 dilution in immunohistochemical analysis followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Cytoplasmic staining on human spleen. The section was incubated with ab252832 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody. Secondary antibody was a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252832).
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for GBP1 using ab252832 at 1/100 dilution in immunohistochemical analysis followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Cytoplasmic staining on human tonsil. The section was incubated with ab252832 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody. Secondary antibody was a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252832).
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ICC/IF analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (+/-IFN gamma (100 U/ml 24h)) cells labeling GBP1 (Green) using ab252832 at 1/30 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Tubulin was counterstained using ab179504 Anti-beta IV Tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 diluton (Red). The nuclear counterstain was DAPI (Blue). Confocal image showing cytoplasmic staining in HeLa cells treated with interferon gamma (100U/ml) for 24 hours.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252832).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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