Immunocytochemistry/ Immunofluorescence abreview for Anti-GATA4 antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Paraffin embedded adult pig heart)
Permeabilization
Yes - 1% Triton X-100, 30' RT
Specification
Paraffin embedded adult pig heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% FBS
Dilution
1/200

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: anti-Rabbit IgG Alexa Fluor­ 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

Additional data

Additional Notes
Green: alpha-actinin 1/250 (EA-53 ab9465) Red: GATA4 1/200 (ab 84593) Blue: Hoechst

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提交于 Sep 13 2017

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