Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA4 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Intestinal epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate Buffer
Permeabilization
Yes - Triton in TBS
Specification
Intestinal epithelium
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 3% BSA + 0.2% Tween
Dilution
1/50

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-Alexa 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568

Additional data

Additional Notes
Paraffin sections- Weak nuclear signal detected in duodenal epithelial cells. No nuclear signal observed in colonic epithelial cells.

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提交于 Jul 11 2017

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