Immunocytochemistry/ Immunofluorescence abreview for Anti-GATA4 antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human embryonic stem cell derived cardiomyocytes a)
Specification
Human embryonic stem cell derived cardiomyocytes a
Fixative
Methanol
Permeabilization
Yes - 0.1% Triton-X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% goat serum

Secondary antibody

Name
Non-Abcam antibody was used: FITC goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/1000

Additional data

Additional Notes
I used this antibody to stain cardiomyocytes, and the affinity was high however the antibody also stained fibroblasts. I have dual stained with cardiac alpha actinin (cy3 labelled) which is specific for cardiomyocytes.

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提交于 Oct 19 2010

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