概述

  • 产品名称

  • 描述

    兔多克隆抗体to GATA4
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Rhesus monkey
    预测可用于: Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human GATA4 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab94555)

  • 阳性对照

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Caco 2.

性能

应用

Our Abpromise guarantee covers the use of ab84593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/50.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

靶标

  • 功能

    Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • 疾病相关

    Atrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 序列相似性

    Contains 2 GATA-type zinc fingers.
  • 翻译后修饰

    Methylation at Lys-300 attenuates transcriptional activity.
  • 细胞定位

    Nucleus.
  • Information by UniProt
  • 数据库链接

  • 别名

    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

图片

  • All lanes : Anti-GATA4 antibody (ab84593) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • ab84593 staining GATA4 (green) in mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized in 1% Triton X-100 and blocked with 10% goat serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 10% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Red - Myh6/7, Blue - DAPI.

    See Abreview

  • Immunofluorescence image of Gata4 (ab84593) on human embryonic stem cell derived cardiomyocytes. The cells were fixed in methanol and permeabilized in 0.1% Triton-X-100. In this image you can see a cardiomyocyte stained in red with a cardiac alpha actinin antibody and both the cardiomyocyte and a fibroblast stained in green with ab84593.

    See Abreview

  • All lanes : Anti-GATA4 antibody (ab84593) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human Fetal Heart - Total Protein Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.

文献

This product has been referenced in:

  • Anamthathmakula P  et al. Steroidogenic Factor 1 (Nr5a1) is Required for Sertoli Cell Survival Post Sex Determination. Sci Rep 9:4452 (2019). Read more (PubMed: 30872705) »
  • Vaez SA  et al. The cardiac niche role in cardiomyocyte differentiation of rat bone marrow-derived stromal cells: comparison between static and microfluidic cell culture methods. EXCLI J 17:762-774 (2018). Read more (PubMed: 30190666) »
See all 28 Publications for this product

客户评价及客户问答

1-10 of 18 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Paraffin embedded adult pig heart)
Permeabilization
Yes - 1% Triton X-100, 30' RT
Specification
Paraffin embedded adult pig heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Sep 13 2017

Application
Western blot
Sample
Pig Tissue lysate - whole (Total heart)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Total heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Sep 13 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Intestinal epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate Buffer
Permeabilization
Yes - Triton in TBS
Specification
Intestinal epithelium
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Jul 11 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Feb 13 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Feb 11 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Sep 19 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Sample
Mouse Tissue sections (mouse embryo)
Specification
mouse embryo
Permeabilization
Yes - 1% triton x-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jan 03 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrition buffer
Sample
Mouse Tissue sections (mouse embryo)
Specification
mouse embryo
Permeabilization
Yes - 1% triton x-100
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Jan 02 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Mouse Cell lysate - nuclear (Gata4 over-expressing undifferentiated mouse ES ce)
Specification
Gata4 over-expressing undifferentiated mouse ES ce
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Ms. Elisa Ponsele

Verified customer

提交于 Dec 20 2013

Answer

Thank you for contacting us. Your credit note ID is xxxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. As requested, I have also issued a free of charge replacement with the order number xxxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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1-10 of 18 Abreviews or Q&A

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