概述

  • 产品名称
  • 描述
    兔多克隆抗体to GARS
  • 宿主
    Rabbit
  • 特异性
    This antibody reacts specifically with 79kDa GARS protein.
  • 经测试应用
    适用于: ICC/IF, IP, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • HEK293 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/3000 - 1/10000.
IP 1/3000 - 1/10000.
WB 1/5000. Predicted molecular weight: 83 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22144914

靶标

  • 相关性
    GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
  • 细胞定位
    Cytoplasm. Mitochondrion.
  • 数据库链接
  • 别名
    • AP 4 A synthetase antibody
    • Charcot Marie Tooth neuropathy 2D antibody
    • Charcot Marie Tooth neuropathy neuronal type D antibody
    • CMT2D antibody
    • Diadenosine tetraphosphate synthetase antibody
    • DSMAV antibody
    • EC 6.1.1.14 antibody
    • Glycine tRNA ligase antibody
    • Glycyl tRNA synthetase antibody
    • GlyRS antibody
    • HMN5 antibody
    • SMAD1 antibody
    see all

图片

  • Anti-GARS antibody (ab42905) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 83 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)

  • ICC/IF image of ab42905 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab42905 staining GARS in murine sciatic nerve tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% goat serum in PBS with 0.5% Triton X-100 for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in diluent) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

文献

This product has been referenced in:
  • Boulos S  et al. Serine 207 phosphorylated lysyl-tRNA synthetase predicts disease-free survival of non-small-cell lung carcinoma. Oncotarget 8:65186-65198 (2017). Read more (PubMed: 29029422) »
  • Niehues S  et al. Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nat Commun 6:7520 (2015). Read more (PubMed: 26138142) »

See all 5 Publications for this product

客户评价及客户问答

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Aug 21 2014

Application
Western blot
Sample
Rat Cell lysate - whole cell (C6 Rat glioma cells)
Loading amount
40 µg
Specification
C6 Rat glioma cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (neuron)
Total protein in input
15 µg
Specification
neuron
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 May 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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