Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (Chinese Hamster Ovary (CHO))
Gel Running Conditions
Non-reduced Denaturing (12% SDS-GEL)
Loading amount
50 µg
Specification
Chinese Hamster Ovary (CHO)
Blocking step
Western Breeze kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 21°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 21°C · Diluent: Buffer from Western Breeze kit
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit antibody from Western Breeze anti-rabb
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
Chemiluminescent
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 34, 27, 24 kDa
Additional data
Abcam response
Thank you for participating in our AbTrial program and for your valuable feedback. We are pleased that this antibody worked well for you on Chinese hamster samples, a species that we have not tested yet. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Pavlos Kotidis
Verified customer
提交于 Mar 23 2020