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重组RabMAb

重组Anti-GAPDH抗体[EPR16891] - Loading Control (ab181602)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (19) Submit a question References (894)

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Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Immunoprecipitation - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Flow Cytometry - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16891] to GAPDH - Loading Control
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Chicken, Human, Zebrafish, African green monkey, Xenopus tropicalis

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概述

  • 产品名称

    Anti-GAPDH抗体[EPR16891] - Loading Control
    参阅全部 GAPDH 一抗
  • 描述

    兔单克隆抗体[EPR16891] to GAPDH - Loading Control
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Chicken, Human, Zebrafish, African green monkey, Xenopus tropicalis
    预测可用于: Rabbit, Fish
  • 免疫原

    Recombinant fragment within Mouse GAPDH aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P16858

  • 阳性对照

    • WB: HeLa, UMNSAH/DF-1, Jurkat, COS-1, RAW 264.7 and PC-12 whole cell lysates; Human fetal brain and heart lysates; Xenopus(X. tropicalis) muscle lysate; Zebrafish lysate; Mouse kidney and spleen lysates; Rat brain lysate. IHC-P: Human transitional cell carcinoma of bladder, Mouse spleen and Rat spleen tissues. ICC/IF: HeLa cells. Flow: Jurkat cells. IP: HeLa whole cell extract
  • 常规说明

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR16891
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease

相关产品

  • Alternative Versions

    • Anti-GAPDH antibody [EPR16891] - BSA and Azide free (ab199553)
    • Alexa Fluor® 647 Anti-GAPDH antibody [EPR16891] (ab201272)
    • Alexa Fluor® 488 Anti-GAPDH antibody [EPR16891] (ab201768)
    • HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
    • Alexa Fluor® 594 Anti-GAPDH antibody [EPR16891] (ab206371)
    • Alexa Fluor® 405 Anti-GAPDH antibody [EPR16891] (ab206372)
    • PE Anti-GAPDH antibody [EPR16891] (ab224004)
    • FITC Anti-GAPDH antibody [EPR16891] (ab224005)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Mouse GAPDH protein (ab202148)
    • Recombinant Human GAPDH protein (ab77109)
    • Recombinant human GAPDH protein (Active) (ab82633)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-SDHB antibody [21A11AE7] (ab14714)
    • Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
    • Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
    • Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

应用

Our Abpromise guarantee covers the use of ab181602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
Flow Cyt 1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/60.

靶标

  • 功能

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • 通路

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 序列相似性

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻译后修饰

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 细胞定位

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 374193 Chicken
    • Entrez Gene: 2597 Human
    • Entrez Gene: 100042025 Mouse
    • Entrez Gene: 14433 Mouse
    • Entrez Gene: 100009074 Rabbit
    • Entrez Gene: 24383 Rat
    • Entrez Gene: 685186 Rat
    • Entrez Gene: 317743 Zebrafish
    • Omim: 138400 Human
    • SwissProt: P00356 Chicken
    • SwissProt: P04406 Human
    • SwissProt: P16858 Mouse
    • SwissProt: P46406 Rabbit
    • SwissProt: P04797 Rat
    • SwissProt: Q5XJ10 Zebrafish
    • Unigene: 544577 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Unigene: 304088 Mouse
    • Unigene: 309092 Mouse
    • Unigene: 317779 Mouse
    • Unigene: 343110 Mouse
    • Unigene: 392463 Mouse
    • Unigene: 392480 Mouse
    • Unigene: 414470 Mouse
    • Unigene: 458138 Mouse
    • Unigene: 458416 Mouse
    • Unigene: 475698 Mouse
    • Unigene: 129558 Rat
    • Unigene: 91450 Rat
    • Unigene: 35640 Zebrafish
    see all
  • 别名

    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
    • BARS-38 antibody
    • cb609 antibody
    • EC 1.2.1.12 antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • glyceraldehyde 3-PDH antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

图片

  • Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution

    Lane 1 : Mouse kidney lysates
    Lane 2 : Mouse spleen lysates
    Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 5 : Rat brain lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    Immunocytochemistry/immunofluorescence staining of 4% paraformaldehyde fixed; 0.1% triton X 100 permeabilized HeLa (human cervix adenocarcinoma) cells labeling GAPDH with ab181602 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/400. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/500) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/500). The negative controls are shown in the bottom middle and right hand panels- for negative control 1 primary antibody (ab181602; 1/500) and secondary antibody (ab150120; 1/500) was used. For negative control 2 primary antibody (ab7291; 1/500) and secondary antibody (ab150077; 1/400) was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasmic staining on lymphocyte of rat spleen is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Immunoprecipitation - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    GAPDH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181602 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab181602 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract.

    Lane 2: PBS instead of HeLa whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Flow Cytometry - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Flow Cytometry - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling GAPDH with ab181602 at 1/180 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/50000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : Xenopus tropicalis muscle lysates
    Lane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic and nucleus staining on the tumor cells of transitional cell carcinoma of human bladder is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution

    Lane 1 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
    Lane 2 : Zebrafish lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal heart lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasmic staining on lymphocytes of mouse spleen is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

实验方案

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • 文献 (894)

    发表研究结果有使用 ab181602?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab181602 被引用在 894 文献中.

    • Gao Z  et al. Regulatory effects of lncRNA ATB targeting miR-200c on proliferation and apoptosis of colorectal cancer cells. J Cell Biochem 121:332-343 (2020). PubMed: 31222825
    • Zhang Y  et al. Mycobacterium tuberculosis Heat-Shock Protein 16.3 Induces Macrophage M2 Polarization Through CCRL2/CX3CR1. Inflammation 43:487-506 (2020). PubMed: 31748849
    • Li X & Zheng H LncRNA SNHG1 influences cell proliferation, migration, invasion, and apoptosis of non-small cell lung cancer cells via the miR-361-3p/FRAT1 axis. Thorac Cancer 11:295-304 (2020). PubMed: 31788970
    • Tutusaus A  et al. A Functional Role of GAS6/TAM in Nonalcoholic Steatohepatitis Progression Implicates AXL as Therapeutic Target. Cell Mol Gastroenterol Hepatol 9:349-368 (2020). PubMed: 31689560
    • Zhu G  et al. MyD88 mediates colorectal cancer cell proliferation, migration and invasion via NF-?B/AP-1 signaling pathway. Int J Mol Med 45:131-140 (2020). PubMed: 31746347
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 19 Abreviews

    Western blot abreview for Anti-GAPDH antibody [EPR16891] - Loading Control

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    Application
    Western blot
    Sample
    Chinese hamster Cell lysate - whole cell (Chinese Hamster Ovary (CHO))
    Gel Running Conditions
    Non-reduced Denaturing (12% SDS-GEL)
    Loading amount
    50 µg
    Specification
    Chinese Hamster Ovary (CHO)
    Blocking step
    Western Breeze kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 21°C
    Read More

    Pavlos Kotidis

    Verified customer

    提交于 Mar 23 2020

    Western blot abreview for Anti-GAPDH antibody [EPR16891] - Loading Control

    Excellent
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    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    10 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 27 2020

    Western blot abreview for Anti-GAPDH antibody [EPR16891] - Loading Control

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    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (L Cells (ATCC® CRL-2648))
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    20 µg
    Specification
    L Cells (ATCC® CRL-2648)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 20 2020

    Western blot abreview for Anti-GAPDH antibody [EPR16891]

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    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (E. coli)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Specification
    E. coli
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Haibo Wang

    Verified customer

    提交于 Feb 27 2018

    Western blot abreview for Anti-GAPDH antibody [EPR16891]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 29 2016

    Western blot abreview for Anti-GAPDH antibody [EPR16891]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain, liver, retina)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    20 µg
    Specification
    Brain, liver, retina
    Blocking step
    Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 25 2016

    Western blot abreview for Anti-GAPDH antibody [EPR16891]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (Brain, retina)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    20 µg
    Specification
    Brain, retina
    Blocking step
    Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 25 2016

    Western blot abreview for Anti-GAPDH antibody [EPR16891]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ARPE19, hfRPE, HEK293)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    20 µg
    Specification
    ARPE19, hfRPE, HEK293
    Blocking step
    Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 25 2016

    Western blot abreview for Anti-GAPDH antibody [EPR16891]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (skin)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    skin
    Blocking step
    Commercial blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 23°C
    Read More

    Maral Tajerian

    Verified customer

    提交于 Jul 15 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GAPDH antibody [EPR16891]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (undifferentiated Human ES cells on MEFs)
    Permeabilization
    Yes - see blocking buffer
    Specification
    undifferentiated Human ES cells on MEFs
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 22 2016

    1-10 of 19 Abreviews

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