Name: Anti-GAPDH antibody [EPR16891] - Loading Control
Brand: RabMAb
SKU: ab181602
Rating: 5 (20 reviews)

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Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16891] to GAPDH - Loading Control
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Chicken, Human, Zebrafish, African green monkey, Xenopus tropicalis

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free FITC HRP PE

产品名称

Anti-GAPDH抗体[EPR16891] - Loading Control
参阅全部 GAPDH 一抗
描述

兔单克隆抗体[EPR16891] to GAPDH - Loading Control
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
种属反应性

与反应: Mouse, Rat, Chicken, Human, Zebrafish, African green monkey, Xenopus tropicalis
预测可用于: Rabbit, Fish
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HeLa, UMNSAH/DF-1, Jurkat, COS-1, RAW 264.7 and PC-12 whole cell lysates; Human fetal brain and heart lysates; Xenopus(X. tropicalis) muscle lysate; Zebrafish lysate; Mouse kidney and spleen lysates; Rat brain lysate. IHC-P: Human transitional cell carcinoma of bladder, Mouse spleen and Rat spleen tissues. ICC/IF: HeLa cells. Flow: Jurkat cells. IP: HeLa whole cell extract
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR16891
同种型

IgG
研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab181602于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/180. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(17)	1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF	(3)	1/500.
IP		1/60.

说明
Flow Cyt (Intra) 1/180. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
IP 1/60.

功能

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
通路

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
序列相似性

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
翻译后修饰

S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.
细胞定位

Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Target information above from: UniProt accession P04406 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 374193 Chicken
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 100009074 Rabbit
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Entrez Gene: 317743 Zebrafish
- Omim: 138400 Human
- SwissProt: P00356 Chicken
- SwissProt: P04406 Human
- SwissProt: P16858 Mouse
- SwissProt: P46406 Rabbit
- SwissProt: P04797 Rat
- SwissProt: Q5XJ10 Zebrafish
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
- Unigene: 304088 Mouse
- Unigene: 309092 Mouse
- Unigene: 317779 Mouse
- Unigene: 343110 Mouse
- Unigene: 392463 Mouse
- Unigene: 392480 Mouse
- Unigene: 414470 Mouse
- Unigene: 458138 Mouse
- Unigene: 458416 Mouse
- Unigene: 475698 Mouse
- Unigene: 129558 Rat
- Unigene: 91450 Rat
- Unigene: 35640 Zebrafish
see all
别名
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- G3PD antibody
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- Glyceraldehyde 3 phosphate dehydrogenase antibody
- glyceraldehyde 3-PDH antibody
- Glyceraldehyde-3-phosphate dehydrogenase antibody
- HEL-S-162eP antibody
- KNC-NDS6 antibody
- MGC102544 antibody
- MGC102546 antibody
- MGC103190 antibody
- MGC103191 antibody
- MGC105239 antibody
- MGC127711 antibody
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- OCAS, p38 component antibody
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see all

Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution

Lane 1 : Mouse kidney lysates
Lane 2 : Mouse spleen lysates
Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 5 : Rat brain lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Immunocytochemistry/immunofluorescence staining of 4% paraformaldehyde fixed; 0.1% triton X 100 permeabilized HeLa (human cervix adenocarcinoma) cells labeling GAPDH with ab181602 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/400. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/500) was used to stain tubulin along with ab150120 (AlexaFluor^®594 goat anti-mouse secondary, 1/500). The negative controls are shown in the bottom middle and right hand panels- for negative control 1 primary antibody (ab181602; 1/500) and secondary antibody (ab150120; 1/500) was used. For negative control 2 primary antibody (ab7291; 1/500) and secondary antibody (ab150077; 1/400) was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasmic staining on lymphocyte of rat spleen is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

GAPDH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181602 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab181602 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract.

Lane 2: PBS instead of HeLa whole cell extract.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling GAPDH with ab181602 at 1/180 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/50000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Xenopus tropicalis muscle lysates
Lane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Different batches of ab181602 were tested on HeLa (Human cervix adenocarcinoma epithelial cell) lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 36 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic and nucleus staining on the tumor cells of transitional cell carcinoma of human bladder is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution

Lane 1 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
Lane 2 : Zebrafish lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GAPDH with ab181602 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasmic staining on lymphocytes of mouse spleen is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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发表研究结果有使用 ab181602？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab181602 被引用在 1134 文献中.

Guo S et al. Ligustilide alleviates the insulin resistance, lipid accumulation, and pathological injury with elevated phosphorylated AMPK level in rats with diabetes mellitus. J Recept Signal Transduct Res 41:85-92 (2021). PubMed: 32643505
Lu W et al. Reprogramming immunosuppressive myeloid cells facilitates immunotherapy for colorectal cancer. EMBO Mol Med 13:e12798 (2021). PubMed: 33283987
Liu Z et al. CircZNF609 promotes cell proliferation, migration, invasion, and glycolysis in nasopharyngeal carcinoma through regulating HRAS via miR-338-3p. Mol Cell Biochem 476:175-186 (2021). PubMed: 32970285
Liang X et al. Vitamin A deficiency indicating as low expression of LRAT may be a novel biomarker of primary hypertension. Clin Exp Hypertens 43:151-163 (2021). PubMed: 33052059
Ye Y et al. Circ-AK2 is associated with preeclampsia and regulates biological behaviors of trophoblast cells through miR-454-3p/THBS2. Placenta 103:156-163 (2021). PubMed: 33129036

View all Publications for this product

客户评价及客户问答

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1-10 of 20 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Lung cancer cells)

Gel Running Conditions

Reduced Denaturing (10-14)

Loading amount

40 µg

Specification

Lung cancer cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Marie Klimontova

Verified customer

提交于 Apr 09 2021

Application

Western blot

Sample

Chinese hamster Cell lysate - whole cell (Chinese Hamster Ovary (CHO))

Gel Running Conditions

Non-reduced Denaturing (12% SDS-GEL)

Loading amount

50 µg

Specification

Chinese Hamster Ovary (CHO)

Blocking step

Western Breeze kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 21°C

Pavlos Kotidis

Verified customer

提交于 Mar 23 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

10 µg

Specification

HEK293

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jan 27 2020

Application

Western blot

Sample

Mouse Cell lysate - whole cell (L Cells (ATCC® CRL-2648))

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

20 µg

Specification

L Cells (ATCC® CRL-2648)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jan 20 2020

Application

Western blot

Sample

Escherichia coli Cell lysate - whole cell (E. coli)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

10 µg

Specification

E. coli

Blocking step

BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Haibo Wang

Verified customer

提交于 Feb 27 2018

Application

Western blot

Sample

Human Cell lysate - whole cell (Fibroblasts)

Gel Running Conditions

Reduced Denaturing

Loading amount

50 µg

Specification

Fibroblasts

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Nov 29 2016

Application

Western blot

Sample

Mouse Tissue lysate - whole (Brain, liver, retina)

Gel Running Conditions

Reduced Denaturing (12% gel)

Loading amount

20 µg

Specification

Brain, liver, retina

Blocking step

Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 25 2016

Application

Western blot

Sample

Zebrafish Tissue lysate - whole (Brain, retina)

Gel Running Conditions

Reduced Denaturing (12% gel)

Loading amount

20 µg

Specification

Brain, retina

Blocking step

Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 25 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (ARPE19, hfRPE, HEK293)

Gel Running Conditions

Reduced Denaturing (12% gel)

Loading amount

20 µg

Specification

ARPE19, hfRPE, HEK293

Blocking step

Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 25 2016

Application

Western blot

Sample

Mouse Tissue lysate - whole (skin)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

skin

Blocking step

Commercial blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 23°C

Maral Tajerian

Verified customer

提交于 Jul 15 2016

1-10 of 20 Abreviews or Q&A

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

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Compatible Secondaries

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Isotype control

Recombinant Protein

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应用

The Abpromise guarantee

靶标

功能

通路

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (1134)

客户评价及客户问答

Western blot abreview for Anti-GAPDH antibody [EPR16891] - Loading Control

Western blot abreview for Anti-GAPDH antibody [EPR16891] - Loading Control

Western blot abreview for Anti-GAPDH antibody [EPR16891] - Loading Control

Western blot abreview for Anti-GAPDH antibody [EPR16891] - Loading Control

Western blot abreview for Anti-GAPDH antibody [EPR16891]

Western blot abreview for Anti-GAPDH antibody [EPR16891]

Western blot abreview for Anti-GAPDH antibody [EPR16891]

Western blot abreview for Anti-GAPDH antibody [EPR16891]

Western blot abreview for Anti-GAPDH antibody [EPR16891]

Western blot abreview for Anti-GAPDH antibody [EPR16891]