Application
Western blot
Sample
Human Cell lysate - whole cell (H1299 cells)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Specification
H1299 cells
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
21 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 680 LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Detection
Detection method
Licor Odyssey Clx
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Positive control
Mouse brain, heart, liver H1299 cells
Additional data
Additional Notes
Lane 1: Mouse brain
Lane 2: Mouse heart
Lane 3: Mouse liver
Lane 4: CD38 Mouse liver
Lane 5: H1299 cells
PVDF membrane RIPA lysis buffer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 21 2018