重组Anti-Galectin 1抗体[EPR3205] (ab108389)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3205] to Galectin 1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Galectin 1抗体[EPR3205]
参阅全部 Galectin 1 一抗 -
描述
兔单克隆抗体[EPR3205] to Galectin 1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HL60, K562, and NIH/3T3 cell lysates. Mouse heart, kidney, and spleen tissue lysates. Rat heart and kidney tissue lysates; ICC/IF: NIH/3T3 and K562 cells. IHC-P: Human placenta tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3205 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108389于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
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ICC/IF |
1/100.
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IHC-P |
1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa. |
ICC/IF
1/100. |
IHC-P
1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
组织特异性
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
序列相似性
Contains 1 galectin domain. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 3956 Human
- Entrez Gene: 16852 Mouse
- Entrez Gene: 56646 Rat
- Omim: 150570 Human
- SwissProt: P09382 Human
- SwissProt: P16045 Mouse
- SwissProt: P11762 Rat
- Unigene: 445351 Human
see all -
别名
- 14 kDa laminin-binding protein antibody
- 14 kDa lectin antibody
- Beta galactoside binding lectin antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded human placenta tissue labelling Galectin 1 with ab108389 at 1/3000 dilution (0.42 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. Positive staining on vessels in human placenta tissue. The section was incubated with ab108389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 mins.
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All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa -
Immunocytochemistry/immunofluorescence analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
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Immunocytochemistry/immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
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All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution
Lane 1 : mouse heart tissue lysate
Lane 2 : mouse kidney tissue lysate
Lane 3 : mouse spleen tissue lysate
Lane 4 : rat heart tissue lysate
Lane 5 : rat kidney tissue lysate
Lane 6 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab108389 被引用在 12 文献中.
- Chiang MT et al. Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation. Arterioscler Thromb Vasc Biol 41:331-345 (2021). PubMed: 33147994
- Lim JY et al. Myeloma-Secreted Galectin-1 Potently Interacts with CD304 on Monocytic Myeloid-Derived Suppressor Cells. Cancer Immunol Res 9:503-513 (2021). PubMed: 33771821
- Chen K et al. Both serum and tissue Galectin-1 levels are associated with adverse clinical features in neuroblastoma. Pediatr Blood Cancer 65:e27229 (2018). PubMed: 29797641
- Gao Y et al. Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer. Cell Death Dis 9:416 (2018). PubMed: 29549328
- Shahbaz S et al. CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-ß. PLoS Biol 16:e2006649 (2018). PubMed: 30550561