Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Squirrel Tissue sections (Brain, preoptic area)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain, preoptic area
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/600
Name
Non-Abcam antibody was used: vector
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
I did more dilution 1:100, 1:400, 1:800
incubation at 4C for 24h or 48h
Abcam response
We would like to thank the reviewer for submitting this Abreview for ab97739 . As the ab97739 had not yet been tested in IHC-FoFr on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing ab97739 in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 20 2017