存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.02% Sodium azide
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Our Abpromise guarantee covers the use of ab1398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1000 - 1/5000.|
|WB||1/500 - 1/1000.|
功能Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
组织特异性Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
序列相似性Belongs to the ATG8 family.
翻译后修饰The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
细胞定位Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
- Information by UniProt
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
Anti-GABARAP antibody (ab1398) at 1 µg/ml + Macrophage whole cell lysate at 80 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 14 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
This product has been referenced in:
- Li L et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . Read more (PubMed: 28534953) »
- Ning HJ et al. Propofol reduces hypoxia-induced autophagic cell death through downregulating HIF 1a in alveolar epithelial type II cells of rats. Mol Med Rep 16:1509-1515 (2017). Read more (PubMed: 28586054) »