概述

  • 产品名称
    Anti-GABARAP抗体
    参阅全部 GABARAP 一抗
  • 描述
    兔多克隆抗体to GABARAP
  • 宿主
    Rabbit
  • 特异性
    This antibody recognizes human GABARAP (14 kD).
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    RSEGEKIRKKYPDRVPV

    , corresponding to amino acids 15-31 of Human GABARAP.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

应用

Our Abpromise guarantee covers the use of ab1398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    Western Blot: use at 1:500-1:1,000 dilution.
    ELISA: use at 1:1,000-1:5,000 dilution

    • 靶标

    • 功能
      Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    • 组织特异性
      Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • 序列相似性
      Belongs to the ATG8 family.
    • 翻译后修饰
      The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
    • 细胞定位
      Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
    • Information by UniProt
    • 数据库链接
      • 别名
        • ATG8A antibody
        • FLC 3B antibody
        • FLC3B antibody
        • FLJ25768 antibody
        • GABA type A receptor associated protein antibody
        • GABA(A) receptor associated protein antibody
        • GABA(A) receptor-associated protein antibody
        • GABARAP a antibody
        • GABARAP antibody
        • Gamma aminobutyric acid receptor associated protein antibody
        • Gamma-aminobutyric acid receptor-associated protein antibody
        • GBRAP_HUMAN antibody
        • MGC120154 antibody
        • MGC120155 antibody
        • MM 46 antibody
        • MM46 antibody
        see all

      图片

      实验方案

      文献

      This product has been referenced in:
      • Li L  et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . Read more (PubMed: 28534953) »
      • Belichenko PV  et al. Excitatory-inhibitory relationship in the fascia dentata in the Ts65Dn mouse model of Down syndrome. J Comp Neurol 512:453-66 (2009). Mouse . Read more (PubMed: 19034952) »

      See all 2 Publications for this product

      发表研究结果有使用 ab1398？请让我们知道，以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

      提交评价 提交问题

      4 reviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-GABARAP antibody

       Excellent
      Abreviews
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Macrophages)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      80 µg
      Specification
      Macrophages
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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      Abcam user community

      Verified customer

      提交于 Apr 12 2017

      What is the specificity of this ab towards Drosophila?

      DISCOUNT CODE: XXXXXXXXXX
      Expiration date: 03-04-2012
      Value: £227

      I am very pleased to hear you would like to accept our offer and test ab1398 in Drosophila. This code will give you £227 off your next order before the expir...

      Read More

      Hi,
      I'm potentially interested in buying an anti-GABARAP antibody for use in immunofluorescent staining. I have found that the sequence of Drosophila melanogaster Atg8a is identical in some regions to that of human GABARAP - are any of your anti-G...

      Read More

      Thank you very much for your interest in our antibodies.

      According to a quick blast check the immunogen of ab1398 has a homology with Drosophila melanogaster of 82%. We generally expect cross reaction of an antibody from 85% homology and mor...

      Read More

      I acquired the antibody ab1398 generated against a synthetic peptide, but I would like to know against which specific sequence was generated. Thank you very much in advance

      Thank you for your enquiry. The immunogen sequence used corresponds to: amino acids 15-31 (RSEGEKIRKKYPDRVPV) of the GABARAP protein. the data sheet has been updated to reflect this information. If you need any further help please do not hesitate to ge...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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