Anti-GABA Transporter 1 / GAT 1抗体(ab426)
Key features and details
- Rabbit polyclonal to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Chicken
- Isotype: IgG
概述
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产品名称
Anti-GABA Transporter 1 / GAT 1抗体
参阅全部 GABA Transporter 1 / GAT 1 一抗 -
描述
兔多克隆抗体to GABA Transporter 1 / GAT 1 -
宿主
Rabbit -
特异性
Reacts specifically with a 72 kDa protein. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Chicken
预测可用于: Human -
免疫原
Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
Database link: P23978 -
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa). |
靶标
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功能
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. -
序列相似性
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. -
结构域
The PDZ domain-binding motif is involved in the interaction with MPP5. -
细胞定位
Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons. - Information by UniProt
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数据库链接
- Entrez Gene: 415960 Chicken
- Entrez Gene: 6529 Human
- Entrez Gene: 232333 Mouse
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P31648 Mouse
- SwissProt: P23978 Rat
see all -
别名
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
图片
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All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
数据表及文件
文献 (24)
ab426 被引用在 24 文献中.
- Guimarães-Souza EM et al. Retinal alterations in a pre-clinical model of an autism spectrum disorder. Mol Autism 10:19 (2019). PubMed: 31011411
- Zhang W et al. Role of microRNA-155 in modifying neuroinflammation and ?-aminobutyric acid transporters in specific central regions after post-ischaemic seizures. J Cell Mol Med 23:5017-5024 (2019). PubMed: 31144434
- Leonetti A et al. Epileptic seizures and oxidative stress in a mouse model over-expressing spermine oxidase. Amino Acids N/A:N/A (2019). PubMed: 31197571
- Ge MM et al. The therapeutic potential of GABA in neuron-glia interactions of cancer-induced bone pain. Eur J Pharmacol 858:172475 (2019). PubMed: 31228456
- Qian Z et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). PubMed: 29693164