概述

  • 产品名称
    Anti-GABA Transporter 1 / GAT 1抗体
    参阅全部 GABA Transporter 1 / GAT 1 一抗
  • 描述
    兔多克隆抗体to GABA Transporter 1 / GAT 1
  • 宿主
    Rabbit
  • 特异性
    Reacts specifically with a 72 kDa protein.
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
    Database link: P23978

  • 阳性对照
    • IHC-Fr: Mouse retinal tissue. WB: Rat hippocampus, spinal cord and brain tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500.
IHC-Fr 1/50 - 1/500.
WB 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能
    Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • 序列相似性
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • 结构域
    The PDZ domain-binding motif is involved in the interaction with MPP5.
  • 细胞定位
    Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

图片

  • ab426 staining the GABA transporter 1 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2000 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

文献

This product has been referenced in:
  • Qian Z  et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). Read more (PubMed: 29693164) »
  • Ding XD  et al. Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Exp Ther Med 13:2783-2786 (2017). Read more (PubMed: 28588664) »
See all 17 Publications for this product

客户评价及客户问答

1-10 of 13 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (Brain_Region B)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
Permeabilization
No
Specification
Brain_Region B
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
formalin

Poonam Rani

Verified customer

提交于 Apr 07 2016

Answer

Vielen Dank für Ihre Anfrage.

Um es gleich zu sagen: Die Chance, dass unsere Antikörper in EM getestet wurden, ist leider relativ gering. Das heißt, wir können die Anwendung nicht garantieren. Ich kann Ihnen aber einen Test- Rabatt ausstellen.
Desweiteren gibt es mehrere Subtypen der GABA A- und B-Rezeptoren sowie des Glycin -Rezeptors. Haben Sie da eine spezielle Vorliebe?

Ich freue mich auf Ihre Antwort.

Read More

Answer

Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Question

Here are answers on your questions about ab72448 using in our WB studies:


Could you tell me the lysis buffer that you have used? RIPA + proteinase inhibitor + PMSF
Have you reduced and denatured your samples? Which method/buffers did you use?
Invitrogen 4x Loading buffer + 10x Reducing agent. Samples were heated for 5 min at 85C.
How much total protein did you use per well? 10 µg/well
Did you load a loading control? Which LC did you use? What were the results?
Loading control – GAPDH, very nice bends
Did you load a positive control? Which PC did you use? What were the results?
Positive control: parietal cortex, rat. The primary Abs: ab426 (Abcam) and anti-Gat-1 (Alomone Labs) shows a strong bend for GAT-1 with concentration of protein 10 µg/well from the positive control. But ab72448 (Abcam) did not show the clear bend for GAT-1 (only very weak signal between 55 and 58 kDa, but not 67kDa as was stated in description for this product).
What membrane type did you transfer to? PVDF membrane
What blocking steps did you use? 5% NF dry milk /TBST
What dilutions of this product did you use? How long and at what temperature was this antibody incubated? Ab72448 (dilution 1:500), overnight at +4C.
What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated? Odyssey (1 hr), goat anti-Rabbit Fluorescent Ab (IRDye680) 1:4000; HRP (1.5 hrs), donkey anti-rabbit HRP (1:4000)
Have you run a no primary antibody control blot? What were the results? We did not.
What is the wash buffer that you use? TBST
Could you provide the Abcam with any images you may have? Yes (on the image: first well (to the right from the ladder) is positive control (parietal cortex, rat), the others are hypothalamic tissue, rat. Loading Control - GAPDH (green).

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Answer

Thank you for your message and for providing this further information.

From our discussion and from reviewing your protocol and images it appears likely that in this case the issue lies in the vial of this product itself. I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement product, refund or credit note in compensation.

I look forward to hearing from you with details of how you would like to proceed.

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Question
Answer

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I am including a brief questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.



Could you tell me the lysis buffer that you have used?

Have you reduced and denatured your samples? Which method/buffers did you use?

How much total protein did you use per well?

Did you load a loading control? Which LC did you use? What were the results?

Did you load a positive control? Which PC did you use? What were the results?

What membrane type did you transfer to?

What blocking steps did you use?

What dilutions of this product did you use? How long and at what temperature was this antibody incubated?

What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated?

Have you run a no primary antibody control blot? What were the results?

What is the wash buffer that you use?

Could you provide the Abcam with any images you may have?

Finally, would you be able to provide the order confirmation number or PO number used to purchase this?

Read More

Answer

Thank you for your reply. As ab426 sold as whole antiserum, the concentration will not be known. Antibody concentration is usually measured using a protein assay. As serum will contain many proteins, this would not provide an accurate result. However, as an estimate, serum is thought to contain about 1 - 10 mg/ml of antibody. With regards to ab72445, the concentration of this antibody is likely to be lot specific. I woudl appreciate if you could confirm the lot number you have, and I can request the concentration for you. I hope this will be helpful, and I look forward to hearing from you.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Loading amount
10 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Mrs. Olga Cravetchi

Verified customer

提交于 Nov 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse retina sections)
Specification
mouse retina sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Mr. Kevin Jin

Verified customer

提交于 Nov 19 2010

Answer

Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody (ab426:Rabbit polyclonal to GABA Transporter 1 / GAT 1) has been tested and characterised for IHC both on frozen and formalin-fixed embedded tissue sections. We have also received a good review stating that ab426 worked nicely on embryionic chick ciliary ganglion. Please take a look at the review section on the datasheet (top panel). If the primary neurons express the target protein, you can use this antibody for IF/ICC. We would suggest fixing the cells in acetone or ethanol rather than in formalin. Try this antibody at 1:100 and adjust accordingly from the results obtained. If you need anything further or any help then please let us know.

Read More

Answer

Thank you for your email. Concerning ab426, we received instructions from our technical department not to send you the vial which we have in stock currently, but to wait for a specific vial from our suppliers. We are still waiting for this vial and will send it to you as soon as possible. Unfortunately, to your dismay, the packaging still got shipped. Sorry for the inconvenience.

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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