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Neuroscience Neurotransmitter Amino Acids GABA

Anti-GABA Transporter 1 / GAT 1抗体(ab426)

  • Datasheet
Reviews (3)Q&A (10)References (24)

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Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)

    Key features and details

    • Rabbit polyclonal to GABA Transporter 1 / GAT 1
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Chicken
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-GABA Transporter 1 / GAT 1抗体
      参阅全部 GABA Transporter 1 / GAT 1 一抗
    • 描述

      兔多克隆抗体to GABA Transporter 1 / GAT 1
    • 宿主

      Rabbit
    • 特异性

      Reacts specifically with a 72 kDa protein.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Rat, Chicken
      预测可用于: Human
    • 免疫原

      Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
      Database link: P23978

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Constituent: Whole serum
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurotransmitter
      • Amino Acids
      • GABA
      • Neuroscience
      • Neurotransmitter
      • Transporters
      • GABA

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    Our Abpromise guarantee covers the use of ab426 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).

    靶标

    • 功能

      Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
    • 序列相似性

      Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
    • 结构域

      The PDZ domain-binding motif is involved in the interaction with MPP5.
    • 细胞定位

      Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
    • Target information above from: UniProt accession P30531 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 415960 Chicken
      • Entrez Gene: 6529 Human
      • Entrez Gene: 232333 Mouse
      • Entrez Gene: 79212 Rat
      • Omim: 137165 Human
      • SwissProt: P30531 Human
      • SwissProt: P31648 Mouse
      • SwissProt: P23978 Rat
      • Unigene: 443874 Human
      • Unigene: 5260 Mouse
      • Unigene: 10035 Rat
      see all
    • 别名

      • GABATHG antibody
      • GABATR antibody
      • GABT 1 antibody
      • GABT1 antibody
      • GAT-1 antibody
      • GAT1 antibody
      • SC6A1_HUMAN antibody
      • Slc6a1 antibody
      • Sodium and chloride dependent GABA transporter 1 antibody
      • Sodium- and chloride-dependent GABA transporter 1 antibody
      • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
      • Solute carrier family 6 member 1 antibody
      see all

    图片

    • Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
      Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
      All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml

      Lane 1 : Rat Hippocampus Tissue Lysate
      Lane 2 : Spinal Cord (Rat) Tissue Lysate
      Lane 3 : Brain (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 67 kDa
      Observed band size: 74 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (24)

    发表研究结果有使用 ab426?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab426 被引用在 24 文献中.

    • Guimarães-Souza EM  et al. Retinal alterations in a pre-clinical model of an autism spectrum disorder. Mol Autism 10:19 (2019). PubMed: 31011411
    • Zhang W  et al. Role of microRNA-155 in modifying neuroinflammation and ?-aminobutyric acid transporters in specific central regions after post-ischaemic seizures. J Cell Mol Med 23:5017-5024 (2019). PubMed: 31144434
    • Leonetti A  et al. Epileptic seizures and oxidative stress in a mouse model over-expressing spermine oxidase. Amino Acids N/A:N/A (2019). PubMed: 31197571
    • Ge MM  et al. The therapeutic potential of GABA in neuron-glia interactions of cancer-induced bone pain. Eur J Pharmacol 858:172475 (2019). PubMed: 31228456
    • Qian Z  et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). PubMed: 29693164
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GABA Transporter 1 / GAT 1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Guinea pig Tissue sections (Brain_Region B)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
    Permeabilization
    No
    Specification
    Brain_Region B
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    formalin
    Read More

    Poonam Rani

    Verified customer

    提交于 Apr 07 2016

    Question

    wir benötigen Primär-Antikörper, die für Elektronenmikroskopie (TEM) am Gehirn der Maus geeignet sind (postembedding immunogold).

    Konkret handelt es sich um folgende nachzuweisende Proteine:
    GABA A-Rezeptor
    GABA B-Rezeptor
    GABA-Transporter (GAT 1 + GAT3)

    Glycin -Rezeptor
    Glycin-Transporter (GlyT1 + GlyT2)

    Welche Antikörper aus Ihrem Sortiment können Sie uns für unsere Anwendung empfehlen.

    Read More

    Abcam community

    Verified customer

    Asked on May 30 2012

    Answer

    Vielen Dank für Ihre Anfrage.

    Um es gleich zu sagen: Die Chance, dass unsere Antikörper in EM getestet wurden, ist leider relativ gering. Das heißt, wir können die Anwendung nicht garantieren. Ich kann Ihnen aber einen Test- Rabatt ausstellen.
    Desweiteren gibt es mehrere Subtypen der GABA A- und B-Rezeptoren sowie des Glycin -Rezeptors. Haben Sie da eine spezielle Vorliebe?

    Ich freue mich auf Ihre Antwort.

    Read More

    Abcam Scientific Support

    回复于 May 30 2012

    Question

    Thank you for your fast respond. This time we would like to get a REFUND. This is the best option for us. When we will be ready we'll buy more antibodies in Abcam.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 04 2012

    Answer

    Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    回复于 Apr 04 2012

    Question

    Here are answers on your questions about ab72448 using in our WB studies:


    Could you tell me the lysis buffer that you have used? RIPA + proteinase inhibitor + PMSF
    Have you reduced and denatured your samples? Which method/buffers did you use?
    Invitrogen 4x Loading buffer + 10x Reducing agent. Samples were heated for 5 min at 85C.
    How much total protein did you use per well? 10 µg/well
    Did you load a loading control? Which LC did you use? What were the results?
    Loading control – GAPDH, very nice bends
    Did you load a positive control? Which PC did you use? What were the results?
    Positive control: parietal cortex, rat. The primary Abs: ab426 (Abcam) and anti-Gat-1 (Alomone Labs) shows a strong bend for GAT-1 with concentration of protein 10 µg/well from the positive control. But ab72448 (Abcam) did not show the clear bend for GAT-1 (only very weak signal between 55 and 58 kDa, but not 67kDa as was stated in description for this product).
    What membrane type did you transfer to? PVDF membrane
    What blocking steps did you use? 5% NF dry milk /TBST
    What dilutions of this product did you use? How long and at what temperature was this antibody incubated? Ab72448 (dilution 1:500), overnight at +4C.
    What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated? Odyssey (1 hr), goat anti-Rabbit Fluorescent Ab (IRDye680) 1:4000; HRP (1.5 hrs), donkey anti-rabbit HRP (1:4000)
    Have you run a no primary antibody control blot? What were the results? We did not.
    What is the wash buffer that you use? TBST
    Could you provide the Abcam with any images you may have? Yes (on the image: first well (to the right from the ladder) is positive control (parietal cortex, rat), the others are hypothalamic tissue, rat. Loading Control - GAPDH (green).

    Read More

    Abcam community

    Verified customer

    Asked on Apr 02 2012

    Answer

    Thank you for your message and for providing this further information.

    From our discussion and from reviewing your protocol and images it appears likely that in this case the issue lies in the vial of this product itself. I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement product, refund or credit note in compensation.

    I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    回复于 Apr 02 2012

    Question

    Called to discuss problems using ab72448.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 30 2012

    Answer

    Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As we discussed on the phone, I am including a brief questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

    I look forward to receiving your reply.



    Could you tell me the lysis buffer that you have used?

    Have you reduced and denatured your samples? Which method/buffers did you use?

    How much total protein did you use per well?

    Did you load a loading control? Which LC did you use? What were the results?

    Did you load a positive control? Which PC did you use? What were the results?

    What membrane type did you transfer to?

    What blocking steps did you use?

    What dilutions of this product did you use? How long and at what temperature was this antibody incubated?

    What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated?

    Have you run a no primary antibody control blot? What were the results?

    What is the wash buffer that you use?

    Could you provide the Abcam with any images you may have?

    Finally, would you be able to provide the order confirmation number or PO number used to purchase this?

    Read More

    Abcam Scientific Support

    回复于 Mar 30 2012

    Question

    Hello, The product codes (catalog number) are the follows:   GABA transporter 1/GAT 1: ab426   GABA A Receptor alpha 2: ab72445   I would be very appreciate if you could provide me the concentrations of these antibodies. Thank you very much, Regards,      

    Read More

    Abcam community

    Verified customer

    Asked on Dec 01 2011

    Answer

    Thank you for your reply. As ab426 sold as whole antiserum, the concentration will not be known. Antibody concentration is usually measured using a protein assay. As serum will contain many proteins, this would not provide an accurate result. However, as an estimate, serum is thought to contain about 1 - 10 mg/ml of antibody. With regards to ab72445, the concentration of this antibody is likely to be lot specific. I woudl appreciate if you could confirm the lot number you have, and I can request the concentration for you. I hope this will be helpful, and I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    回复于 Dec 01 2011

    Western blot abreview for Anti-GABA Transporter 1 / GAT 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Loading amount
    10 µg
    Specification
    brain
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More

    Mrs. Olga Cravetchi

    Verified customer

    提交于 Nov 22 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-GABA Transporter 1 / GAT 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse retina sections)
    Specification
    mouse retina sections
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Mr. Kevin Jin

    Verified customer

    提交于 Nov 19 2010

    Question

    PRE-SALES ENQUIRY Concerning ab426, the rabbit anti-GAT1 antibody, has this been shown to work in immunocytochemistry/immunofluorescence of primary neurons? And if so, what dilution and in what conditions? Formaldehyde fixed with glutaraldehyde or just formaldehyde-fixed samples? Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 30 2004

    Answer

    Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody (ab426:Rabbit polyclonal to GABA Transporter 1 / GAT 1) has been tested and characterised for IHC both on frozen and formalin-fixed embedded tissue sections. We have also received a good review stating that ab426 worked nicely on embryionic chick ciliary ganglion. Please take a look at the review section on the datasheet (top panel). If the primary neurons express the target protein, you can use this antibody for IF/ICC. We would suggest fixing the cells in acetone or ethanol rather than in formalin. Try this antibody at 1:100 and adjust accordingly from the results obtained. If you need anything further or any help then please let us know.

    Read More

    Abcam Scientific Support

    回复于 Sep 01 2004

    Question

    today we received your package. Unfortunately there were only a cup, some ice and some papers in, but no antibody. Please could you send us the antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 23 2004

    Answer

    Thank you for your email. Concerning ab426, we received instructions from our technical department not to send you the vial which we have in stock currently, but to wait for a specific vial from our suppliers. We are still waiting for this vial and will send it to you as soon as possible. Unfortunately, to your dismay, the packaging still got shipped. Sorry for the inconvenience.

    Read More

    Abcam Scientific Support

    回复于 Mar 24 2004

    1-10 of 13 Abreviews or Q&A

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