Anti-FUT11抗体(ab121411)
Key features and details
- Rabbit polyclonal to FUT11
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-FUT11抗体 -
描述
兔多克隆抗体to FUT11 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to C terminal amino acids 348-482 of Human FUT11.
-
阳性对照
- Human liver tissue; Transfected HEK293T Cell lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121411于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/250 - 1/500.
|
说明 |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
靶标
-
功能
Probable fucosyltransferase. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the glycosyltransferase 10 family. -
细胞定位
Golgi apparatus > Golgi stack membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 170384 Human
- SwissProt: Q495W5 Human
- Unigene: 588854 Human
-
别名
- 3)-fucosyltransferase 11 antibody
- Alpha (1,3) fucosyltransferase 11 antibody
- Alpha-(1 antibody
see all
图片
-
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclear membrane and golgi apparatus. Recommended concentration of ab121411 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
ab121411 at 1/50 dilution staining FUT11 in paraffin-embedded Human liver tissue by Immunohistochemistry.
-
All lanes : Anti-FUT11 antibody (ab121411) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : FUT11-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab121411 被引用在 1 文献中.
- Cao W et al. Hypoxia-Related Gene FUT11 Promotes Pancreatic Cancer Progression by Maintaining the Stability of PDK1. Front Oncol 11:675991 (2021). PubMed: 34221996