Anti-FUSIP1抗体- C-terminal (ab173216)

概述

  • 产品名称
    Anti-FUSIP1抗体- C-terminal
    参阅全部 FUSIP1 一抗
  • 描述
    兔多克隆抗体to FUSIP1 - C-terminal
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide within Human SR Proteins aa 196-225 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O75494

  • 阳性对照
    • 293 cell line lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab173216 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab173216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 31 kDa.

靶标

  • 功能
    Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰
    Phosphorylated. Fully dephosphorylated in mitosis and partially dephosphorylated on heat shock. Isoform 3 is phosphorylated on Ser-168.
  • 细胞定位
    Nucleus speckle. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 40 kDa SR repressor protein antibody
    • 40 kDa SR-repressor protein antibody
    • Anti TLS associated protein with SR repeats antibody
    • arginine/serine-rich 13A antibody
    • FUS interacting protein serine arginine rich 1 antibody
    • FUS interacting protein serine arginine rich 2 antibody
    • FUS interacting serine arginine rich protein 1 antibody
    • FUS-interacting serine-arginine-rich protein 1 antibody
    • FUSIP1 antibody
    • FUSIP2 antibody
    • NSSR antibody
    • OTTHUMP00000015774 antibody
    • Serine arginine repressor protein 40 kDa antibody
    • Serine/arginine-rich splicing factor 10 antibody
    • SFRS13 antibody
    • SFRS13A antibody
    • Splicing factor antibody
    • Splicing factor arginine serine rich 13 antibody
    • Splicing factor SRp38 antibody
    • SRp38 antibody
    • SRp40 antibody
    • SRrp40 antibody
    • SRS10_HUMAN antibody
    • Srsf10 antibody
    • TASR antibody
    • TASR1 antibody
    • TASR2 antibody
    • TLS associated protein TASR1 antibody
    • TLS associated protein TASR2 antibody
    • TLS associated protein with Ser Arg repeats antibody
    • TLS associated protein with SR repeats antibody
    • TLS associated serine arginine protein 1 antibody
    • TLS associated serine arginine protein 2 antibody
    • TLS associated serine arginine protein antibody
    • TLS associated SR protein antibody
    • TLS-associated protein with Ser-Arg repeats antibody
    • TLS-associated protein with SR repeats antibody
    • TLS-associated serine-arginine protein antibody
    • TLS-associated SR protein antibody
    see all

图片

  • Anti-FUSIP1 antibody - C-terminal (ab173216) at 1/100 dilution + 293 cell line lysate at 35 µg

    Predicted band size: 31 kDa

实验方案

文献

ab173216 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Permeabilization
Yes - 0.3% Triton
Specification
MEF
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 08 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing (10% PAM)
Loading amount
40 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 08 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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