概述

  • 产品名称
    Anti-FRMD1抗体
  • 描述
    兔多克隆抗体to FRMD1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: RYFEPHSYFP QWIITKRGID YILRHMPTLH RERQGLSPKE AMLCFIQEAC RLEDVPVHFF RLHKDKKEGR PTVILGLALR GVHIYQGKKL EI, corresponding to amino acids 205-296 of Human FRMD1.

  • 阳性对照
    • Human bone marrow tissue Human plasma

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 序列相似性
    Contains 1 FERM domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA164L23.1 antibody
    • DKFZp434O0117 antibody
    • FERM domain containing 1 antibody
    • FERM domain-containing protein 1 antibody
    • FLJ00181 antibody
    • FLJ22615 antibody
    • FLJ40260 antibody
    • FRMD1 antibody
    • FRMD1_HUMAN antibody
    • OTTHUMP00000219079 antibody
    • OTTHUMP00000219098 antibody
    • RP11-164L23.1 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (actin filaments). Recommended concentration of ab122109 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-FRMD1 antibody (ab122109) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab122109, at 1/20 staining FRMD1 in paraffin embedded Human bone marrow by Immunohistochemistry.

文献

ab122109 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (Saos2 Human Osteosarcoma)
Specification
Saos2 Human Osteosarcoma
Permeabilization
Yes - TBST
Fixative
Paraformaldehyde

Jonathan Larochelle

Verified customer

提交于 Apr 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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