概述

  • 产品名称

    Free Fatty Acid Assay试剂盒- Quantification
  • 检测方法

    Colorimetric/Fluorometric
  • 样品类型

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型

    Quantitative
  • 灵敏度

    > 2 µM
  • 检测时间

    0h 40m
  • 产品概述

    Free Fatty Acid Assay Kit ab65341 uses a convenient, sensitive enzyme-based method for detecting long-chain free fatty acids in various mammalian and other samples, such as serum, plasma and other body fluids, food, growth media, etc.


    In the free fatty acid assay protocol, fatty acids are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (λ= 570 nm) or fluorometric (Ex/Em= 535/587 nm) methods. Palmitic acid is used to generate a standard curve.


    Free fatty acid assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 30 min at 37ºC
    - analyze with microplate reader

  • 说明

    Previously called Free Fatty Acid Quantification Assay Kit.

    This assay detects C-8 (octanoate) and longer fatty acids.

    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • 平台

    Microplate reader

性能

图片

  • Lipolysis were evaluated by measuring free fatty acids released into the culture medium of siCreg1 or si-Scramble 3T3-L1 cells using ab65341, which were treated with BMS-345541 (5 µmol/L), an NF-?B pathway inhibitor, or vehicle (DMSO) for 48 hours prior to lipolysis assessment. ns, no significance; and ***P<0.001.

  • Plasma FFA levels was measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • Free Fatty Acid measured in biologicals showing concentration (µM)

  • Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

实验方案

文献

This product has been referenced in:

  • Becares N  et al. Impaired LXRa Phosphorylation Attenuates Progression of Fatty Liver Disease. Cell Rep 26:984-995.e6 (2019). Read more (PubMed: 30673619) »
  • Scopelliti A  et al. A Neuronal Relay Mediates a Nutrient Responsive Gut/Fat Body Axis Regulating Energy Homeostasis in Adult Drosophila. Cell Metab 29:269-284.e10 (2019). Read more (PubMed: 30344016) »
See all 74 Publications for this product

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Plasma from yellow-bellied marmots (Marmota flaviventris) was processed according the protocol. A serial dilution of the plasma (shown) was compared to the standard curved (also shown) to determine the optimal dilution factor for subsequent assays.

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提交于 Jul 20 2016

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