Free Fatty Acid Assay试剂盒- Quantification (ab65341)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 60 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
概述
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产品名称
Free Fatty Acid Assay试剂盒- Quantification
参阅全部 Free Fatty Acid 试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 2 µM -
检测时间
0h 60m -
产品概述
Free Fatty Acid Assay Kit ab65341 uses a convenient, sensitive enzyme-based method for detecting long-chain free fatty acids in various mammalian and other samples, such as serum, plasma and other body fluids, food, growth media, etc.
In the free fatty acid assay protocol, fatty acids are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (λ= 570 nm) or fluorometric (Ex/Em= 535/587 nm) methods. Palmitic acid is used to generate a standard curve.
Free fatty acid assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader
Δ Note: Assay time does not include preparation of the samples.
Chinese protocol available. See protocols section below.
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说明
Previously called Free Fatty Acid Quantification Assay Kit.
This assay detects C-8 (octanoate) and longer fatty acids.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
How other researchers have used Free Fatty Acid Assay Kit ab65341
This Free Fatty Acid assay kit has been used in publications in a variety of sample types, including:
- Human: cell culture lysates1, serum2
- Mouse: plasma and liver tissue3, serum4, plasma5, heart tissue6, liver7
- Rat: serum8, plasma9, cytosol of articular chondrocyte primary cell cultures10
- Cell culture medium11
- C reinhardtii algae12
- Drosophila13
- C elegans14References: 1 - Ali A et al 2018, Phokrai P et al 2018; 2 - Tahapary DL et al 2018; 3 - Becares et al 2019; 4 - Pan J et al 2019, Maatta J et al 2018, Rohm M et al 2018, Shimazu T et al 2018; 5 - Woo M et al 2018, Tian X et al 2017; 6 - Liu W et al 2017; 7 - Hao et al 2017, Rui W et al 2016; 8 - Honore SM et al 2018; 9 - Van der Werf et al 2018;10 - Lee SW et al 2018; 11 - Kostrzewski T et al 2017; 12 - Ramanan R et al 2018; 13 - Scopelliti A et al 2019; 14 - Pollard AK et al 2019
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 100 tests ACS Reagent Blue 1 vial Enhancer Purple 1 x 200µl Fatty Acid Assay Buffer WM 1 x 25ml Fatty Acid enzyme mix Green 1 vial Fatty Acid Probe (in DMSO) Red 1 x 200µl Palmitic Acid Standard (1nmol/µl) Yellow 1 x 300µl -
研究领域
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相关性
Fatty acids are carboxylic acids with long hydrocarbon chains. The hydrocarbon chain length may vary from 8-30 carbons. Fatty acids play very important roles in normal metabolism and many disease development. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. -
别名
- FFA
相关产品
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Related Products
图片
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Functional Studies - Free Fatty Acid Assay Kit - Quantification (ab65341)Tian, Xiaoxiang et al., PloS one vol. 12,5 e0176873., Fig 6, doi:10.1371/journal.pone.0176873
Lipolysis were evaluated by measuring free fatty acids released into the culture medium of siCreg1 or si-Scramble 3T3-L1 cells using ab65341, which were treated with BMS-345541 (5 µmol/L), an NF-κB pathway inhibitor, or vehicle (DMSO) for 48 hours prior to lipolysis assessment. ns, no significance; and ***P<0.001.
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Functional studies - ab65341Samuel P et al., PLoS One 8(1), Fig 7a. DOI: 10.1371/journal.pone.0048425. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Plasma FFA levels were measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).
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Func S-1Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
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ab65341 Free Fatty Acid Quantification KitFree Fatty Acid measured in biologicals showing concentration (µM).
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ab65341 Free Fatty Acid Quantification KitFlourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
数据表及文件
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SDS download
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Datasheet download
文献 (134)
ab65341 被引用在 134 文献中.
- Sohlang MN & Majaw S Altered VDAC-HK association and apoptosis in mouse peripheral blood lymphocytes exposed to diabetic condition: an in vitro and in vivo study. Arch Physiol Biochem N/A:1-11 (2021). PubMed: 33434071
- Tangseefa P et al. The mTORC1 complex in pre-osteoblasts regulates whole-body energy metabolism independently of osteocalcin. Bone Res 9:10 (2021). PubMed: 33551450
- Perrin-Cocon L et al. A hexokinase isoenzyme switch in human liver cancer cells promotes lipogenesis and enhances innate immunity. Commun Biol 4:217 (2021). PubMed: 33594203
- Bullock J et al. Degradation of Photoreceptor Outer Segments by the Retinal Pigment Epithelium Requires Pigment Epithelium-Derived Factor Receptor (PEDF-R). Invest Ophthalmol Vis Sci 62:30 (2021). PubMed: 33605986
- Frasca D et al. Influence of obesity on serum levels of SARS-CoV-2-specific antibodies in COVID-19 patients. PLoS One 16:e0245424 (2021). PubMed: 33760825