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Free Fatty Acid Quantification Assay Kit ab65341 provides a convenient, sensitive enzyme-based method for detecting long-chain free fatty acids (FA) in various mammalian and other samples, such as serum, plasma and other body fluids, food, growth media, etc.
In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)
Visit our FAQs page for tips and troubleshooting.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
This assay detects formation of C-8 (octanoate) and longer fatty acids.
|ACS Reagent||Blue||1 vial|
|Enhancer||Purple||1 x 200µl|
|Fatty Acid Assay Buffer||WM||1 x 25ml|
|Fatty Acid enzyme mix||Green||1 vial|
|Fatty Acid Probe (in DMSO)||Red||1 x 200µl|
|Palmitic Acid Standard (1nmol/µl)||Yellow||1 x 300µl|
Plasma FFA levels was measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).
Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
Free Fatty Acid measured in biologicals showing concentration (µM)
Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"