Free Fatty Acid Assay试剂盒- Quantification (ab65341)
概述
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产品名称Free Fatty Acid Assay试剂盒- Quantification
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检测方法Colorimetric/Fluorometric
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样品类型Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
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检测类型Quantitative
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灵敏度> 2 µM
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检测时间0h 40m
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产品概述
Free Fatty Acid Assay Kit ab65341 uses a convenient, sensitive enzyme-based method for detecting long-chain free fatty acids in various mammalian and other samples, such as serum, plasma and other body fluids, food, growth media, etc.
In the free fatty acid assay protocol, fatty acids are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (λ= 570 nm) or fluorometric (Ex/Em= 535/587 nm) methods. Palmitic acid is used to generate a standard curve.
Free fatty acid assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader -
说明
Previously called Free Fatty Acid Quantification Assay Kit.
This assay detects C-8 (octanoate) and longer fatty acids.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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平台Microplate reader
性能
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存放说明Store at -20°C. Please refer to protocols.
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组件 标识符 100 tests ACS Reagent Blue 1 vial Enhancer Purple 1 x 200µl Fatty Acid Assay Buffer WM 1 x 25ml Fatty Acid enzyme mix Green 1 vial Fatty Acid Probe (in DMSO) Red 1 x 200µl Palmitic Acid Standard (1nmol/µl) Yellow 1 x 300µl -
研究领域
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相关性Fatty acids are carboxylic acids with long hydrocarbon chains. The hydrocarbon chain length may vary from 8-30 carbons. Fatty acids play very important roles in normal metabolism and many disease development. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response.
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图片
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Functional Studies - Free Fatty Acid Assay Kit - Quantification (ab65341)Tian, Xiaoxiang et al., PloS one vol. 12,5 e0176873., Fig 6, doi:10.1371/journal.pone.0176873Lipolysis were evaluated by measuring free fatty acids released into the culture medium of siCreg1 or si-Scramble 3T3-L1 cells using ab65341, which were treated with BMS-345541 (5 µmol/L), an NF-?B pathway inhibitor, or vehicle (DMSO) for 48 hours prior to lipolysis assessment. ns, no significance; and ***P<0.001.
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Functional studies - ab65341Samuel P et al., PLoS One 8(1), Fig 7a. DOI: 10.1371/journal.pone.0048425.Plasma FFA levels was measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).
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Func S-1Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
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ab65341 Free Fatty Acid Quantification KitFree Fatty Acid measured in biologicals showing concentration (µM)
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ab65341 Free Fatty Acid Quantification KitFlourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
实验方案
文献
This product has been referenced in:
- Becares N et al. Impaired LXRa Phosphorylation Attenuates Progression of Fatty Liver Disease. Cell Rep 26:984-995.e6 (2019). Read more (PubMed: 30673619) »
- Scopelliti A et al. A Neuronal Relay Mediates a Nutrient Responsive Gut/Fat Body Axis Regulating Energy Homeostasis in Adult Drosophila. Cell Metab 29:269-284.e10 (2019). Read more (PubMed: 30344016) »
