Free Fatty Acid Assay试剂盒- Quantification (ab65341)

Abreviews (1)Q&A (33)Specific References (35)

概述

  • 产品名称
    Free Fatty Acid Assay试剂盒- Quantification
    参阅全部 Fatty acid 试剂盒
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型
    Quantitative
  • 灵敏度
    > 2 µM
  • 检测时间
    0h 40m
  • 产品概述

    Free Fatty Acid Quantification Assay Kit ab65341 provides a convenient, sensitive enzyme-based method for detecting long-chain free fatty acids (FA) in various mammalian and other samples, such as serum, plasma and other body fluids, food, growth media, etc.


    In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)



    Visit our FAQs page for tips and troubleshooting.


    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • 说明

    This assay detects formation of C-8 (octanoate) and longer fatty acids.

性能

图片

  • Functional studies - ab65341
    Functional studies - ab65341Samuel P et al., PLoS One 8(1), Fig 7a. DOI: 10.1371/journal.pone.0048425.

    Plasma FFA levels was measured using ab65341 in male and female wild-type (WT) or AT2KO mice either on normal diet (ND) or high fat diet (HFD).

  • Func S-1
    Func S-1

    Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

  • ab65341 Free Fatty Acid Quantification Kit
    ab65341 Free Fatty Acid Quantification Kit

    Free Fatty Acid measured in biologicals showing concentration (µM)

  • ab65341 Free Fatty Acid Quantification Kit
    ab65341 Free Fatty Acid Quantification Kit

    Flourometric standard curve: mean of duplicates (+/-SD) with background readings subtracted.

实验方案

文献

This product has been referenced in:
  • Harm S  et al. Removal of stabilizers from human serum albumin by adsorbents and dialysis used in blood purification. PLoS One 13:e0191741 (2018). Human . Read more (PubMed: 29364955) »
  • Shimazu T  et al. Role of METTL20 in regulating ß-oxidation and heat production in mice under fasting or ketogenic conditions. Sci Rep 8:1179 (2018). Read more (PubMed: 29352221) »

See all 35 Publications for this product

发表研究结果有使用 ab65341？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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34 reviews or Q&A

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Does tissue need to be fresh or can we snap freeze in liquid nitrogen and grind a small amount prior to homogenising in chloroform/Tx100?



Although we would suggest to use fresh tissue preferably, tissue that is snap-frozen in liquid nitrogen can be used too. The tissue can be ground and then lipid extraction can be done with choloroform-triton.

Validation of FFA quantification kit for yellow-bellied marmots

 Excellent Good 4/5 (Ease of Use)
Abreviews
Plasma from yellow-bellied marmots (Marmota flaviventris) was processed according the protocol. A serial dilution of the plasma (shown) was compared to the standard curved (also shown) to determine the optimal dilution factor for subsequent assays.
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提交于 Jul 20 2016

Does the kit detect triglycerides or other molecules that have a fatty acid component?

No, the kit does not detect triglycerides or other molecules that have a fatty acid components. The triglycerides need an enzyme to convert them to free fatty acids and this is not present in any of the kit components.

Why is the assay not compatible with heparinized samples?

The reason heparinized plasma is not recommended for the free fatty acid assay is because heparin releases lipolytic enzymes that lead to an overestimation of FFA.

How would I account for endogenous long-chain Acyl-CoAs that are already in my tissue extract samples?

You can test for endogoenous Acyl-CoAs in your samples by including sample wells without added ACS Reagent. In these wells, the long-chain free fatty acids will not be converted to coenzyme A, so if there are any interfering compounds including endogen...

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Is it okay to use this kit with store human serum (-80C)?

We would recommend fresh serum samples for best results. If fresh samples is not an option storage at -80 °C is less likely to degrade the sample quality.

For this product ab65341, blood needs to be collected using an anticoagulant such as...

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I am interested in the Free Fatty Acid Quantification Kit (ab65341). It says to be suitable for the plasma. My question is if this kit suitable for the mouse plasma containing heparin?

Blood needs to be collected using an anticoagulant such as EDTA, sodium citrate, sodium fluoride, or ammonium oxalate. Heparinized plasma is not the best choice as heparin could interfere with the assay.

What is the pH of the Assay Buffer in the kit?

The details of the assay buffer is considered proprietary information. I can give you a pH range. It is between 7.5-8.2






Will it be suitable for lipoxin quantification?


This kit is good for C-8 or longer fatty acids so it should be suitable for measuring lipoxins.

Is C-7 fatty acid suitable for this kit?



Our scientists tried this assay with C7 and C6 fatty acids, but found that the final efficiency of detection decreases by ˜50% in that case.

Therefore I can confirm that the minimal length of suitable fatty acid is C8.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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