Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA PH 9
Permeabilization
Yes - Tween
Specification
Melanoma lymph node
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
no additional fixation
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-T 5% donkey serum
Concentration
10 µg/ml
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
This antibody was stained and imaged for Multiplexed Ion Beam Imaging by Time Of Flight (MIBI-TOF). Staining was performed as described with a metal conjugate of this antibody.
Images were acquired using MIBIscope (Ionpath, Inc.)
Foxp3-Green.
CD4-Red.
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提交于 Apr 21 2021