Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (NSCLC)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 ( 5 min 1000W, 50 min 100W)
Permeabilization
No
Specification
NSCLC
Blocking step
Akoya Biosciences OPAL 7 color Blocking solution as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
45 minute(s) · Temperature: 25°C · Diluent: Zytomed Systems AB Diluent
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Akoya Biosciences HRP-Polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
multispectral Imaging using Akoya Biosciences OPAL 7 color manual kit with anti-FOXP3 @ 5th position of mIHC panel (=5x AR) and OPAL 620 TSA (1-150 dilution, 10 min. reaction)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Sebastian Marwitz
Verified customer
提交于 Apr 20 2020