Application
Immunohistochemistry (Frozen sections)
Sample
Zebra finch Tissue sections (brain)
Permeabilization
Yes - 0.3% Triton-X
Specification
brain
Blocking step
Serum as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 0.3% Triton-X in PBS with 2% NRS
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: (rabbit) anti-goat Alexa 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
This antibody worked extremely well against brain tissue in zebra finch. Perfusion with PLP fixative (4% paraformaldehyde in 0.1 M PB with 0.01 M sodium periodate and 0.1 M L-lysine) postfixation for ~18 hours. Sections permeabilized with 0.3% Triton-X. Frozen sections stained with secondary antibody for fluorescence. Picture shows medium spiny neurons in medial striatum.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sarah Bottjer
Verified customer
提交于 Sep 23 2021