Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Xenopus laevis Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - triton X
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Donkey Serum/0.3% Triton/TBS
Dilution
1/1000
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti Goat AlexaFluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Additional Step: Incubated sections prior to primary with 50mM Ammonium Chloride/TBS for 10min
The procedure worked well for tadpole brains, but have not worked with adult brains yet.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 21 2018