Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (e12.5 mouse embryonic spinal cord)
Specification
e12.5 mouse embryonic spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% horse serum, 0.1% Triton-X100 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti sheep (& goat)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/2000
Additional data
Additional Notes
The donkey anti-sheep used here as secondary antibody strongly crossreacts with goat primary antibodies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Frederic Clotman
Verified customer
提交于 Sep 17 2010