Anti-FOXP2抗体- C-terminal (ab1307)
概述
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产品名称
Anti-FOXP2抗体- C-terminal
参阅全部 FOXP2 一抗 -
描述
山羊多克隆抗体to FOXP2 - C-terminal -
宿主
Goat -
特异性
This antibody is expected to recognise all three reported isoforms (NP_055306.1; NP_683696.2; NP_683697.1). -
经测试应用
适用于: WB, IHC-P, IHC - Wholemount, ICC/IF, IHC-Frmore details -
种属反应性
与反应: Mouse, Human, Zebra finch
预测可用于: Rat, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla
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免疫原
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阳性对照
- WB: Whole tissue lysate from human cerebellum. IHC-Fr: e12.5 mouse embryonic spinal cord tissue. IHC-P: Human testis tissue. ICC/IF: Mouse e12.5 thoracic spinal cord cells. IHC-wholemount: Mouse brain tissue.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab1307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79.9 kDa). | |
| IHC-P | Use at an assay dependent concentration. PubMed: 16901978 | |
| IHC - Wholemount | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 23967072 | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20484636 |
靶标
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功能
Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language. -
组织特异性
Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung. -
疾病相关
Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2). -
序列相似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
发展阶段
Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber. -
结构域
The leucine-zipper is required for dimerization and transcriptional repression. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 449627 Chimpanzee
- Entrez Gene: 101150977 Gorilla
- Entrez Gene: 93986 Human
- Entrez Gene: 114142 Mouse
- Entrez Gene: 500037 Rat
- Entrez Gene: 613237 Rhesus monkey
- Entrez Gene: 734154 Xenopus laevis
- Omim: 605317 Human
see all -
别名
- CAG repeat protein 44 antibody
- CAGH44 antibody
- DKFZp686H1726 antibody
see all
图片
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Western blot - Anti-FOXP2 antibody - C-terminal (ab1307)Anti-FOXP2 antibody - C-terminal (ab1307) at 2 µg/ml + Human cerebellum whole cell lysate
Developed using the ECL technique.
Predicted band size: 79.9 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody - C-terminal (ab1307)Paraffin-embedded human testis tissue stained for FOXP2 with ab1307 at 5 µg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6. AP staining.
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Immunohistochemistry (Frozen sections) - Anti-FOXP2 antibody - C-terminal (ab1307)Image courtesy of Dr F Clotman by Abreview.ab1307 staining FOXP2 in e12.5 mouse embryonic spinal cord tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 10% serum for 30 minutes at 20°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 16 hours at 4°C. The secondary antibody used was a donkey anti-sheep (and goat) IgG conjugated to Alexa Fluor® 594 (red) used at a 1/2000 dilution.
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IHC - Wholemount - Anti-FOXP2 antibody - C-terminal (ab1307)Abreview from Verfied customerMouse brain tissue stained for FOXP2 with ab1307 (1/1000 dilution) in immunohistochemical analysis.
Brain explant taken from E15 embryo and cultured for 1 month. Fixed for 4 hours in 4% PFA, permeabilised with PBS-TritonX 100 (0,25%). FOXP2 antibody (ab1307) was used at 1/1000 and a monoclonal mouse antibody was used to visualize CaBP. Secondary antibody incubated at RT for 2 hours.
Image shows and overlay of FoxP2-positive deep cerebellar nuclei neurons (red) and CaBP-positive Purkinje cells (green). -
Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody - C-terminal (ab1307)Francius C et al, PLoS One. 2013 Aug 15;8(8):e70325. doi: 10.1371/journal.pone.0070325. Figure 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Mouse e12.5 thoracic spinal cord stained for FOXP2 (green) using ab1307 (1/2000 dilution) in ICC/IF.
实验方案
数据表及文件
文献
This product has been referenced in:
- Burkett ZD et al. FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch. Elife 7:N/A (2018). Read more (PubMed: 29360038) »
- Fong WL et al. Differential and Overlapping Pattern of Foxp1 and Foxp2 Expression in the Striatum of Adult Mouse Brain. Neuroscience 388:214-223 (2018). Read more (PubMed: 30031127) »
