Key features and details
- Rabbit polyclonal to FOXN3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
参阅全部 FOXN3 一抗
经测试应用适用于: WB, IPmore details
预测可用于: Rabbit, Horse, Chicken, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to Human FOXN3 aa 440-490.
Database link: NP_001078940.1
- WB: Jurkat, HEK-293T and HeLa whole cell lysates.
This product was previously labelled as CHES1
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab129453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
功能Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).
序列相似性Contains 1 fork-head DNA-binding domain.
- Information by UniProt
- C14orf116 antibody
- Checkpoint suppressor 1 antibody
- CHES1 antibody
All lanes : Anti-FOXN3 antibody (ab129453) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutes
Detection of FOXN3 by Western Blot of Immunprecipitate.
Lane 1: ab129453 at 1µg/ml staining FOXN3 in HeLa whole cell lysate immunoprecipitated using ab129453 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
ab129453 被引用在 4 文献中.
- He H et al. Novel tumor-suppressor FOXN3 is downregulated in adult acute myeloid leukemia. Oncol Lett 18:1521-1529 (2019). PubMed: 31423219
- Xu Z et al. Checkpoint suppressor 1 suppresses transcriptional activity of ERa and breast cancer cell proliferation via deacetylase SIRT1. Cell Death Dis 9:559 (2018). PubMed: 29752474
- Sun M et al. MicroRNA-378 regulates epithelial-mesenchymal transition and metastasis of melanoma by inhibiting FOXN3 expression through the Wnt/ß-catenin pathway. Cell Biol Int N/A:N/A (2018). PubMed: 29972255
- Li W et al. The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. J Clin Invest 127:3421-3440 (2017). PubMed: 28805661