Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Ovary)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Ovary
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Anti-goat
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Antigen retrieval: Protease (Sigma#P5147 10mg) in PBS (20ml) for 5mins.
Destroy endogenous peroxidase activity: 0.3% H2O2/methanol solution for 30 mins.
Chromagen: AEC (Sigma#A6926) used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 14 2015