Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Specification
Kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-0.1%Tween
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey-anti-goat AF647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
We did observe some staining, but difficult to assess if it was specific or not. Also a lot of background staining
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this species we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this species. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 01 2018